Book Volume 1071 2014

Fluorescent Protein-Based Biosensors

Methods and Protocols

Editors:

ISBN: 978-1-62703-621-4 (Print) 978-1-62703-622-1 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Protocol

    Pages 1-16

    The Design and Application of Genetically Encodable Biosensors Based on Fluorescent Proteins

  3. No Access

    Protocol

    Pages 17-28

    An Introduction to Fluorescence Imaging Techniques Geared Towards Biosensor Applications

  4. No Access

    Protocol

    Pages 29-47

    Quantitative Measurement of Ca2+ and Zn2+ in Mammalian Cells Using Genetically Encoded Fluorescent Biosensors

  5. No Access

    Protocol

    Pages 49-58

    Detecting cAMP with an Epac-Based FRET Sensor in Single Living Cells

  6. No Access

    Protocol

    Pages 59-71

    Analysis of Compartmentalized cAMP: A Method to Compare Signals from Differently Targeted FRET Reporters

  7. No Access

    Protocol

    Pages 73-81

    Genetically Encoded Fluorescent Biosensors for Live Cell Imaging of Lipid Dynamics

  8. No Access

    Protocol

    Pages 83-95

    Live-Cell Imaging of Cytosolic NADH–NAD+ Redox State Using a Genetically Encoded Fluorescent Biosensor

  9. No Access

    Protocol

    Pages 97-108

    Measuring Membrane Voltage with Microbial Rhodopsins

  10. No Access

    Protocol

    Pages 109-128

    Imaging the Activity of Ras Superfamily GTPase Proteins in Small Subcellular Compartments in Neurons

  11. No Access

    Protocol

    Pages 129-137

    Imaging Kinase Activity at Protein Scaffolds

  12. No Access

    Protocol

    Pages 139-149

    Using a Genetically Encoded FRET-Based Reporter to Visualize Calcineurin Phosphatase Activity in Living Cells

  13. No Access

    Protocol

    Pages 151-161

    Genetically Encoded FRET Indicators for Live-Cell Imaging of Histone Acetylation

  14. No Access

    Protocol

    Pages 163-174

    Genetically Encoded Fluorescent Biosensors for Live-Cell Imaging of MT1-MMP Protease Activity

  15. No Access

    Protocol

    Pages 175-194

    Biosensor Imaging in Brain Slice Preparations

  16. No Access

    Protocol

    Pages 195-206

    Optical Calcium Imaging Using DNA-Encoded Fluorescence Sensors in Transgenic Fruit Flies, Drosophila melanogaster

  17. No Access

    Protocol

    Pages 207-215

    A Multiparameter Live Cell Imaging Approach to Monitor Cyclic AMP and Protein Kinase A Dynamics in Parallel

  18. No Access

    Protocol

    Pages 217-225

    FRET and BRET-Based Biosensors in Live Cell Compound Screens

  19. No Access

    Protocol

    Pages 227-248

    Integrating Fluorescent Biosensor Data Using Computational Models

  20. Back Matter

    Pages 249-251