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The Inflammasome

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Christine M. De Nardo

    1. , Institute of Innate Immunity, University of Bonn, Bonn, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Eicke Latz

    1. , Institute of Innate Immunity, University of Bonn, Bonn, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Presents current up-to-date methodologies
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1040)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Assessing β-Amyloid-Induced NLRP3 Inflammasome Activation in Primary Microglia

    • Mareike Schnaars, Hannes Beckert, Annett Halle
    Pages 1-8

  3. Activating the NLRP3 Inflammasome Using the Amyloidogenic Peptide IAPP

    • Clara Westwell-Roper, Aisling Dunne, Man Lyang Kim, C. Bruce Verchere, Seth L. Masters
    Pages 9-18

  4. Assessment and Quantification of Crystal-Induced Lysosomal Damage

    • Peter Duewell, Eicke Latz
    Pages 19-27

  5. Assessment of Inflammasome Activation in Primary Human Immune Cells

    • Theo S. Plantinga, Leo A. B. Joosten, Mihai G. Netea
    Pages 29-39

  6. NLRP3 Inflammasome Activation and Cytotoxicity Induced by Particulate Adjuvants

    • Marie Yang, Claire H. A. Hearnden, Ewa Oleszycka, Ed C. Lavelle
    Pages 41-63

  7. Measuring Inflammasome Activation in Response to Bacterial Infection

    • Petr Broz, Denise M. Monack
    Pages 65-84

  8. Detection of Pyroptosis by Measuring Released Lactate Dehydrogenase Activity

    • Manira Rayamajhi, Yue Zhang, Edward A. Miao
    Pages 85-90

  9. ASC Speck Formation as a Readout for Inflammasome Activation

    • Andrea Stutz, Gabor L. Horvath, Brian G. Monks, Eicke Latz
    Pages 91-101

  10. Immunoblotting for Active Caspase-1

    • Christopher Jakobs, Eva Bartok, Andrej Kubarenko, Franz Bauernfeind, Veit Hornung
    Pages 103-115

  11. Inflammasome Activation and Inhibition in Primary Murine Bone Marrow-Derived Cells, and Assays for IL-1α, IL-1β, and Caspase-1

    • Katharina S. Schneider, Christina J. Thomas, Olaf Groß
    Pages 117-135

  12. Reconstituting the NLRP1 Inflammasome In Vitro

    • Benjamin Faustin, John C. Reed
    Pages 137-152

  13. Assessing ATP Binding and Hydrolysis by NLR Proteins

    • Jinyao Mo, Joseph A. Duncan
    Pages 153-168

  14. Blue Native Polyacrylamide Gel Electrophoresis to Monitor Inflammasome Assembly and Composition

    • Eric M. Kofoed, Russell E. Vance
    Pages 169-183

  15. Analysis of Microbiota Alterations in Inflammasome-Deficient Mice

    • Eran Elinav, Christoph A. Thaiss, Richard A. Flavell
    Pages 185-194

  16. Quantification of Adipose Tissue Leukocytosis in Obesity

    • Ryan Grant, Yun-Hee Youm, Anthony Ravussin, Vishwa Deep Dixit
    Pages 195-209

  17. In Vivo Evaluation of Neutrophil Recruitment in Response to Sterile Particulates

    • Tamiko Yanagida, Kaoru Orihashi, Hajime Kono
    Pages 211-221

  18. Back Matter

    Pages 223-225
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Keywords

About this book

 Inflammasomes have a seemingly simple architecture. A sensor molecule of the NLR or PYHIN protein family recruits the common adapter molecule apoptosis-associated speck-like protein containing a CARD (ASC). Even so, inflammasome activation is highly regulated at multiple steps and precise details of how inflammasomes are activated at the molecular level remain largely unknown. In The Inflammasome: Methods and Protocols, expert researchers in the field detail methods and protocols to study multiple aspects of inflammasome function. These methods include the generation of inflammasome stimuli, perturbance of cellular homeostasis, assessment of ASC speck formation, monitoring of caspase-1 activity and processing, activation of IL-1β cytokines, and analysis of inflammasome assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Editors and Affiliations

  • , Institute of Innate Immunity, University of Bonn, Bonn, Germany

    Christine M. De Nardo, Eicke Latz

Bibliographic Information

  • Book Title: The Inflammasome

  • Book Subtitle: Methods and Protocols

  • Editors: Christine M. De Nardo, Eicke Latz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-523-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-522-4Published: 13 July 2013

  • Softcover ISBN: 978-1-4939-6011-8Published: 23 August 2016

  • eBook ISBN: 978-1-62703-523-1Published: 13 July 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 225

  • Number of Illustrations: 23 b/w illustrations, 21 illustrations in colour

  • Topics: Immunology, Cytokines and Growth Factors

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