Glycosylation Engineering of Biopharmaceuticals

Methods and Protocols

Editors:

ISBN: 978-1-62703-326-8 (Print) 978-1-62703-327-5 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xv

  2. Glyco-Engineering of Therapeutic Proteins

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-17

      Engineering of Therapeutic and Diagnostic O-Glycans on Recombinant Mucin-Type Immunoglobulin Fusion Proteins Expressed in CHO Cells

    3. No Access

      Book Protocol

      Pages 19-29

      Engineering a Human-Like Glycosylation to Produce Therapeutic Glycoproteins Based on 6-Linked Sialylation in CHO Cells

    4. No Access

      Book Protocol

      Pages 31-43

      Glycoengineered Pichia-Based Expression of Monoclonal Antibodies

    5. No Access

      Book Protocol

      Pages 45-57

      N-Glycosylation Humanization for Production of Therapeutic Recombinant Glycoproteins in Saccharomyces cerevisiae

    6. No Access

      Book Protocol

      Pages 59-77

      Engineering the Baculovirus Genome to Produce Galactosylated Antibodies in Lepidopteran Cells

  3. Glycoanalytics

    1. Front Matter

      Pages 79-79

    2. No Access

      Book Protocol

      Pages 81-91

      NanoLC Chips MS/MS for the Characterization of N-Glycopeptides Generated from Trypsin Digestion of a Monoclonal Antibody

    3. No Access

      Book Protocol

      Pages 93-113

      Cetuximab Fab and Fc N-Glycan Fast Characterization Using IdeS Digestion and Liquid Chromatography Coupled to Electrospray Ionization Mass Spectrometry

    4. No Access

      Book Protocol

      Pages 115-143

      Therapeutic Antibody Glycosylation Analysis: A Contract Research Organization Perspective in the Frame of Batch Release or Comparability Support

    5. No Access

      Book Protocol

      Pages 145-167

      Mass Spectrometric Analysis of O-Linked Oligosaccharides from Various Recombinant Expression Systems

    6. No Access

      Book Protocol

      Pages 169-180

      Assessing Fc Glycan Heterogeneity of Therapeutic Recombinant Monoclonal Antibodies Using NP-HPLC

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      Book Protocol

      Pages 181-197

      Application of Capillary Electrophoresis in Glycoprotein Analysis

    8. No Access

      Book Protocol

      Pages 199-209

      Characterization of Glycoprotein Biopharmaceutical Products by Caliper LC90 CE-SDS Gel Technology

    9. No Access

      Book Protocol

      Pages 211-219

      Hydrophobic Interaction Chromatography to Analyze Glycoproteins

    10. No Access

      Book Protocol

      Pages 221-226

      Lectin Glycoprofiling of Recombinant Therapeutic Interleukin-7

    11. No Access

      Book Protocol

      Pages 227-240

      Analysis of Monoclonal Antibodies by Sedimentation Velocity Analytical Ultracentrifugation

  4. Glycoprotein Complexes Characterization

    1. Front Matter

      Pages 241-241

    2. No Access

      Book Protocol

      Pages 243-268

      Noncovalent Mass Spectrometry for the Characterization of Antibody/Antigen Complexes

    3. No Access

      Book Protocol

      Pages 269-289

      Conformational Analysis of Recombinant Monoclonal Antibodies with Hydrogen/Deuterium Exchange Mass Spectrometry

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      Book Protocol

      Pages 291-302

      Epitope Mapping of Antibodies by Mass Spectroscopy: A Case Study

  5. PK/PD Assays for Therapeutic Antibodies

    1. Front Matter

      Pages 303-303

    2. No Access

      Book Protocol

      Pages 305-317

      Evaluation of Antibody-Dependent Cell Cytotoxicity Using Lactate Dehydrogenase (LDH) Measurement

    3. No Access

      Book Protocol

      Pages 319-329

      Evaluation of Complement-Dependent Cytotoxicity Using ATP Measurement and C1q/C4b Binding

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