Synthetic Messenger RNA and Cell Metabolism Modulation

Methods and Protocols

Editors:

ISBN: 978-1-62703-259-9 (Print) 978-1-62703-260-5 (Online)

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi

  2. mRNA Synthesis, Purification, and Transfer

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-28

      Cell Engineering with Synthetic Messenger RNA

    3. No Access

      Book Protocol

      Pages 29-42

      In Vitro Transcription of Long RNA Containing Modified Nucleosides

    4. No Access

      Book Protocol

      Pages 43-54

      HPLC Purification of In Vitro Transcribed Long RNA

    5. No Access

      Book Protocol

      Pages 55-72

      Synthetic mRNAs with Superior Translation and Stability Properties

    6. No Access

      Book Protocol

      Pages 73-88

      Nonviral, Cationic Lipid-Mediated Delivery of mRNA

    7. No Access

      Book Protocol

      Pages 89-100

      Light-Induced mRNA Transfection

    8. No Access

      Book Protocol

      Pages 101-110

      Engineering B Cells with mRNA

    9. No Access

      Book Protocol

      Pages 111-125

      Programming Human Dendritic Cells with mRNA

    10. No Access

      Book Protocol

      Pages 127-138

      Large Volume Flow Electroporation of mRNA: Clinical Scale Process

    11. No Access

      Book Protocol

      Pages 139-161

      Retrovirus-Based mRNA Transfer for Transient Cell Manipulation

    12. No Access

      Book Protocol

      Pages 163-184

      mRNA Delivery to Human Dendritic Cells by Recombinant Yeast and Activation of Antigen-Specific Memory T Cells

  3. Modulation and monitoring of cell metabolism

    1. Front Matter

      Pages 185-185

    2. No Access

      Book Protocol

      Pages 187-201

      Nonviral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy

    3. No Access

      Book Protocol

      Pages 203-220

      Natural Killer Cell Reprogramming with Chimeric Immune Receptors

    4. No Access

      Book Protocol

      Pages 221-233

      Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA

    5. No Access

      Book Protocol

      Pages 235-246

      Antitumor Vaccination with Synthetic mRNA: Strategies for In Vitro and In Vivo Preclinical Studies

    6. No Access

      Book Protocol

      Pages 247-274

      Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA

    7. No Access

      Book Protocol

      Pages 275-292

      Analysis of Survivin-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA

    8. No Access

      Book Protocol

      Pages 293-303

      mRNA Electroporation as a Tool for Immunomonitoring

    9. No Access

      Book Protocol

      Pages 305-320

      mRNA PCR-Based Epitope Chase Method

  4. Back Matter

    Pages 321-323