Nanotechnology for Nucleic Acid Delivery

Methods and Protocols

Editors:

ISBN: 978-1-62703-139-4 (Print) 978-1-62703-140-0 (Online)

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xii

  2. No Access

    Book Protocol

    Pages 1-12

    Toxicological Aspects for Nanomaterial in Humans

  3. No Access

    Book Protocol

    Pages 13-22

    Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity

  4. No Access

    Book Protocol

    Pages 23-33

    Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity

  5. No Access

    Book Protocol

    Pages 35-47

    Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery

  6. No Access

    Book Protocol

    Pages 49-65

    Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers In Vivo

  7. No Access

    Book Protocol

    Pages 67-84

    Lipids for Nucleic Acid Delivery: Synthesis and Particle Formation

  8. No Access

    Book Protocol

    Pages 85-103

    Histidine-Rich Cationic Amphipathic Peptides for Plasmid DNA and siRNA Delivery

  9. No Access

    Book Protocol

    Pages 105-120

    Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids

  10. No Access

    Book Protocol

    Pages 121-132

    Synthesis of Bioreducible Polycations with Controlled Topologies

  11. No Access

    Book Protocol

    Pages 133-147

    Lyophilization of Synthetic Gene Carriers

  12. No Access

    Book Protocol

    Pages 149-169

    Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles

  13. No Access

    Book Protocol

    Pages 171-182

    Layer-by-Layer Assembled Gold Nanoparticles for the Delivery of Nucleic Acids

  14. No Access

    Book Protocol

    Pages 183-193

    In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films

  15. No Access

    Book Protocol

    Pages 195-204

    Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles

  16. No Access

    Book Protocol

    Pages 205-241

    Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery

  17. No Access

    Book Protocol

    Pages 243-250

    Synthesis of Lipidic Magnetic Nanoparticles for Nucleic Acid Delivery

  18. No Access

    Book Protocol

    Pages 251-262

    Lipopeptide Delivery of siRNA to the Central Nervous System

  19. No Access

    Book Protocol

    Pages 263-273

    Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery

  20. No Access

    Book Protocol

    Pages 275-284

    Liver-Targeted Gene Delivery Through Retrograde Intrabiliary Infusion

  21. Back Matter

    Pages 285-286