Table of contents (19 protocols)

1. Front Matter

   Pages i-xii

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2. 

   Book Protocol

   Pages 1-12

   Toxicological Aspects for Nanomaterial in Humans

   • Maria Dusinska,
   • Zuzana Magdolenova
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3. 

   Book Protocol

   Pages 13-22

   Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity

   • Ladan Parhamifar,
   • Helene Andersen
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4. 

   Book Protocol

   Pages 23-33

   Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity

   • Anna K. Larsen,
   • Arnaldur Hall,
   • Henrik Lundsgart
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5. 

   Book Protocol

   Pages 35-47

   Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery

   • Masako Ichihara,
   • Naoto Moriyoshi
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6. 

   Book Protocol

   Pages 49-65

   Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers In Vivo

   • Claire Rome,
   • Julien Gravier,
   • Marie Morille
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7. 

   Book Protocol

   Pages 67-84

   Lipids for Nucleic Acid Delivery: Synthesis and Particle Formation

   • Michel Bessodes,
   • Nathalie Mignet
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8. 

   Book Protocol

   Pages 85-103

   Histidine-Rich Cationic Amphipathic Peptides for Plasmid DNA and siRNA Delivery

   • Antoine Kichler,
   • A. James Mason
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9. 

   Book Protocol

   Pages 105-120

   Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids

   • Wolfgang Rödl,
   • David Schaffert,
   • Ernst Wagner
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10. 

    Book Protocol

    Pages 121-132

    Synthesis of Bioreducible Polycations with Controlled Topologies

    • Ye-Zi You,
    • Jun-Jie Yan,
    • Zhi-Qiang Yu
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11. 

    Book Protocol

    Pages 133-147

    Lyophilization of Synthetic Gene Carriers

    • Julia Christina Kasper,
    • Sarah Küchler
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12. 

    Book Protocol

    Pages 149-169

    Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles

    • Angela K. Pannier,
    • Tatiana Segura
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13. 

    Book Protocol

    Pages 171-182

    Layer-by-Layer Assembled Gold Nanoparticles for the Delivery of Nucleic Acids

    • Eva-Christina Wurster,
    • Asmaa Elbakry
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14. 

    Book Protocol

    Pages 183-193

    In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films

    • Yi Zou,
    • Lei Wan,
    • Jenifer Blacklock
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15. 

    Book Protocol

    Pages 195-204

    Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles

    • Steven K. Cool,
    • Bart Geers,
    • Ine Lentacker
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16. 

    Book Protocol

    Pages 205-241

    Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery

    • Dialechti Vlaskou,
    • Christian Plank
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17. 

    Book Protocol

    Pages 243-250

    Synthesis of Lipidic Magnetic Nanoparticles for Nucleic Acid Delivery

    • Yoshihisa Namiki
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18. 

    Book Protocol

    Pages 251-262

    Lipopeptide Delivery of siRNA to the Central Nervous System

    • Mark D. Zabel
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19. 

    Book Protocol

    Pages 263-273

    Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery

    • Olivia M. Merkel,
    • Leigh M. Marsh,
    • Holger Garn
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20. 

    Book Protocol

    Pages 275-284

    Liver-Targeted Gene Delivery Through Retrograde Intrabiliary Infusion

    • Xuan Jiang,
    • Yong Ren,
    • John-Michael Williford
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21. Back Matter

    Pages 285-286

    Download PDF (235KB)