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Calcium Signaling Protocols

  • Book
  • © 2013

Overview

Editors:
  1. David G. Lambert

    1. , Department of Cardiovascular Sciences, Leicester Royal Infirmary, Leicester, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Richard D. Rainbow

    1. , Department of Cardiovascular Sciences, Leicester Royal Infirmary, Leicester, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Combines brand new protocols with heavily revised and improved methodologies
  • Features lab-ready procedures with step-by-step instruction
  • Includes expert tips and key implementation advice to ensure successful results
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 937)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
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  2. General

    1. Front Matter

      Pages 1-1
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    2. Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations

      • Alec W. M. Simpson
      Pages 3-36

    3. Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2

      • Anish Patel, Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, David G. Lambert
      Pages 37-47

  3. Specialist Measurement Systems

    1. Front Matter

      Pages 49-49
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    2. Confocal Microscopy: Theory and Applications for Cellular Signaling

      • Stephen C. Tovey, Paul J. Brighton, Edward T. W. Bampton, Yan Huang, Gary B. Willars
      Pages 51-93

    3. Ratiometric Ca2+ Measurements Using the FlexStation®Scanning Fluorometer

      • Ian C. B. Marshall, Izzy Boyfield, Shaun McNulty
      Pages 95-101

    4. Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR®)

      • Ian C. B. Marshall, Davina E. Owen, Shaun McNulty
      Pages 103-109

    5. Ratiometric [Ca2+]i Measurements in Adherent Cell-Lines Using the NOVOstar Microplate Reader

      • Benjamin D. Hunt, David G. Lambert
      Pages 111-120

  4. Measurement of Ca2+ Channel Activity

    1. Front Matter

      Pages 121-121
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    2. Whole-Cell Patch-Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterologous Expression Systems and Neurones

      • Jon Brown, Atticus H. Hainsworth, Alessandro Stefani, Andrew D. Randall
      Pages 123-148

    3. Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells

      • Richard D. Rainbow
      Pages 149-160

  5. Measurement of Ins(1,4,5)P3 and Ca2+ Release from Intracellular Stores

    1. Front Matter

      Pages 161-161
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  7. Specialist Measurement Techniques

    1. Front Matter

      Pages 205-205
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    2. Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry

      • Hideo Kanaide, Katsuya Hirano
      Pages 207-216

    3. Calcium Measurements from Whole Heart Using Rhod-2

      • Bum-Rak Choi
      Pages 217-228

    4. Measurement of Changes in Endothelial and Smooth Muscle Ca2+ in Pressurized Arteries

      • Kim A. Dora, Michael A. Hill
      Pages 229-238
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Keywords

About this book

The regulation of intracellular Ca2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued.  With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca2+ regulation and provided much new information on processes.  Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca2+ signalling for screening, and genetically encoded sensors.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca2+.

Editors and Affiliations

  • , Department of Cardiovascular Sciences, Leicester Royal Infirmary, Leicester, United Kingdom

    David G. Lambert, Richard D. Rainbow

Bibliographic Information

  • Book Title: Calcium Signaling Protocols

  • Editors: David G. Lambert, Richard D. Rainbow

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-086-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-085-4Published: 26 September 2012

  • Softcover ISBN: 978-1-4939-5928-0Published: 23 August 2016

  • eBook ISBN: 978-1-62703-086-1Published: 25 September 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 360

  • Topics: Biochemistry, general

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