Table of contents (29 protocols)

1. Front Matter

   Pages i-xiv

   Download PDF (336KB)

2. 

   Book Protocol

   Pages 1-20

   Overview of Molecular Biological Methods for the Detection of Pathogens Causing Sexually Transmitted Infections

   • Fernando Vazquez,
   • Luis Otero,
   • Santiago Melón
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3. 

   Book Protocol

   Pages 21-34

   Guidelines for the Qualitative Detection of Viral Genomes in Dried Blood Spots

   • Davide Gibellini,
   • Elisa De Crignis
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4. 

   Book Protocol

   Pages 35-50

   Guidelines for the Quantification of HIV and HCV in Small Volume Whole Blood Samples

   • Tony Bruns,
   • Katrin Steinmetzer
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5. 

   Book Protocol

   Pages 51-64

   Guidelines for High-Resolution Genotyping of Chlamydia trachomatis Using Multilocus Sequence Analysis

   • Linus Christerson,
   • Björn Herrmann
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6. 

   Book Protocol

   Pages 65-101

   Guidelines for the Use of Molecular Tests for the Detection and Genotyping of Human Papilloma Virus from Clinical Specimens

   • Sin Hang Lee
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7. 

   Book Protocol

   Pages 103-112

   The Molecular Diagnosis of Sexually Transmitted Genital Ulcer Disease

   • Cheng-Yen Chen,
   • Ronald C. Ballard
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8. 

   Book Protocol

   Pages 113-128

   Validation of a Sensitive and Specific Real-Time PCR for Detection and Quantitation of Hepatitis B Virus Covalently Closed Circular DNA in Plasma of Chronic Hepatitis B Patients

   • R. B. Takkenberg,
   • S. Menting
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9. 

   Book Protocol

   Pages 129-139

   Protocol for the Detection of Mycoplasma genitalium by PCR from Clinical Specimens and Subsequent Detection of Macrolide Resistance-Mediating Mutations in Region V of the 23S rRNA Gene

   • Jørgen Skov Jensen
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10. 

    Book Protocol

    Pages 141-167

    Protocols for Detection and Typing of Treponema pallidum Using PCR Methods

    • S. M. Bruisten
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11. 

    Book Protocol

    Pages 169-175

    Extraction of DNA from Dried Blood in the Diagnosis of Congenital CMV Infection

    • Jutte J. C. de Vries,
    • Maria Barbi
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12. 

    Book Protocol

    Pages 177-193

    Protocol for the Use of PCR-Denaturing Gradient Gel Electrophoresis and Quantitative PCR to Determine Vaginal Microflora Constitution and Pathogens in Bacterial Vaginosis

    • Beatrice Vitali,
    • Elena Biagi
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13. 

    Book Protocol

    Pages 195-204

    Protocol for the Use of a Bead Array for the Multiple Detection of Genotype of Chlamydia trachomatis

    • Chung-Te Huang,
    • Shu-Ying Li
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14. 

    Book Protocol

    Pages 205-223

    Protocol for the Detection and Genotyping of Human Papillomaviruses Using a Liquid Bead Microarray Assay

    • Stephen Cherne,
    • Viorica Popov,
    • Qinghua Feng
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15. 

    Book Protocol

    Pages 225-233

    Protocol for the Use of Enzyme-Linked Hybridization Assays for Genital Ulcer Disease

    • Seweryn Bialasiewicz,
    • Ian M. Mackay
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16. 

    Book Protocol

    Pages 235-253

    Protocol for the Rapid Detection of the Urogenital Tract Mollicutes and Chlamydia with Concomitant LGV-(sub)typing

    • Nadine Möbius,
    • Wibke Brenneisen
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17. 

    Book Protocol

    Pages 255-261

    Protocol for the Use of Self-Reporting Duplex Mutation Primers to Detect PCR Products in the Diagnosis of HBV

    • Qian-Feng Xia
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18. 

    Book Protocol

    Pages 263-271

    Protocol for the Use of a Rapid Real-Time PCR Method for the Detection of HIV-1 Proviral DNA Using Double-Stranded Primer

    • Chou-Pong Pau,
    • Susan K. Wells
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19. 

    Book Protocol

    Pages 273-282

    Protocol for the Use of Light Upon Extension Real-Time PCR for the Determination of Viral Load in HBV Infection

    • Guimin Li,
    • Wangfeng Li,
    • Lixia Liu
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20. 

    Book Protocol

    Pages 283-293

    Protocol for the Use of a Silica Nanoparticle-Enhanced Microcantilever Sensor-Based Method to Detect HBV at Femtomolar Concentrations

    • Sang-Myung Lee,
    • Kyo Seon Hwang
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21. 

    Book Protocol

    Pages 295-306

    Protocol for the Detection of Treponema pallidum in Paraffin-Embedded Specimens

    • Cheng-Yen Chen,
    • Allan Pillay
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