Diagnosis of Sexually Transmitted Diseases

Methods and Protocols

Editors:

ISBN: 978-1-61779-936-5 (Print) 978-1-61779-937-2 (Online)

Table of contents (29 protocols)

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  1. Front Matter

    Pages i-xiv

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    Pages 1-20

    Overview of Molecular Biological Methods for the Detection of Pathogens Causing Sexually Transmitted Infections

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    Pages 21-34

    Guidelines for the Qualitative Detection of Viral Genomes in Dried Blood Spots

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    Pages 35-50

    Guidelines for the Quantification of HIV and HCV in Small Volume Whole Blood Samples

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    Pages 51-64

    Guidelines for High-Resolution Genotyping of Chlamydia trachomatis Using Multilocus Sequence Analysis

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    Pages 65-101

    Guidelines for the Use of Molecular Tests for the Detection and Genotyping of Human Papilloma Virus from Clinical Specimens

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    Pages 103-112

    The Molecular Diagnosis of Sexually Transmitted Genital Ulcer Disease

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    Pages 113-128

    Validation of a Sensitive and Specific Real-Time PCR for Detection and Quantitation of Hepatitis B Virus Covalently Closed Circular DNA in Plasma of Chronic Hepatitis B Patients

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    Pages 129-139

    Protocol for the Detection of Mycoplasma genitalium by PCR from Clinical Specimens and Subsequent Detection of Macrolide Resistance-Mediating Mutations in Region V of the 23S rRNA Gene

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    Pages 141-167

    Protocols for Detection and Typing of Treponema pallidum Using PCR Methods

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    Pages 169-175

    Extraction of DNA from Dried Blood in the Diagnosis of Congenital CMV Infection

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    Pages 177-193

    Protocol for the Use of PCR-Denaturing Gradient Gel Electrophoresis and Quantitative PCR to Determine Vaginal Microflora Constitution and Pathogens in Bacterial Vaginosis

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    Pages 195-204

    Protocol for the Use of a Bead Array for the Multiple Detection of Genotype of Chlamydia trachomatis

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    Pages 205-223

    Protocol for the Detection and Genotyping of Human Papillomaviruses Using a Liquid Bead Microarray Assay

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    Pages 225-233

    Protocol for the Use of Enzyme-Linked Hybridization Assays for Genital Ulcer Disease

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    Pages 235-253

    Protocol for the Rapid Detection of the Urogenital Tract Mollicutes and Chlamydia with Concomitant LGV-(sub)typing

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    Pages 255-261

    Protocol for the Use of Self-Reporting Duplex Mutation Primers to Detect PCR Products in the Diagnosis of HBV

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    Pages 263-271

    Protocol for the Use of a Rapid Real-Time PCR Method for the Detection of HIV-1 Proviral DNA Using Double-Stranded Primer

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    Pages 273-282

    Protocol for the Use of Light Upon Extension Real-Time PCR for the Determination of Viral Load in HBV Infection

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    Pages 283-293

    Protocol for the Use of a Silica Nanoparticle-Enhanced Microcantilever Sensor-Based Method to Detect HBV at Femtomolar Concentrations

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    Pages 295-306

    Protocol for the Detection of Treponema pallidum in Paraffin-Embedded Specimens

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