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Transgenic Plants

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Jim M. Dunwell

    1. School of Biological Sciences, University of Reading, Reading, Berkshire, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  2. Andy C. Wetten

    1. Dept. of Agricultural Botany, School of Biological Sciences, University of Reading, Reading, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Provides access to the most up-to-date improvements being made for the production and analysis of transgenic plants
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 847)

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Table of contents (37 protocols)

  1. Front Matter

    Pages i-xv
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  2. Selection and Detection Methods

    1. Front Matter

      Pages 1-1
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  4. Transformation Technology: Lower Plants

    1. Front Matter

      Pages 33-33
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  5. TRANSFORMATION TECHNOLOGY – LOWER PLANTS

    1. Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii

      • Juliane Neupert, Ning Shao, Yinghong Lu, Ralph Bock
      Pages 35-47

  6. Transformation Technology: Rice

    1. Front Matter

      Pages 49-49
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  7. TRANSFORMATION TECHNOLOGY – RICE

    1. A High-Efficiency Agrobacterium-Mediated Transformation System of Rice (Oryza sativa L.)

      • Kenjirou Ozawa
      Pages 51-57

    2. Selection of Transgenic Rice Plants Using a Herbicide Tolerant Form of the Acetolactate Synthase Gene

      • Masaki Endo, Tsutomu Shimizu, Seiichi Toki
      Pages 59-66

    3. Visual Selection in Rice: A Strategy for the Efficient Identification of Transgenic Calli Accumulating Transgene Products

      • Hiroaki Saika, Haruko Onodera, Seiichi Toki
      Pages 67-74

    4. Characterization of Rice Genes Using a Heterologous Full-Length cDNA Expression System

      • Mieko Higuchi, Youichi Kondou, Masaki Mori, Takanari Ichikawa, Minami Matsui
      Pages 75-90

    5. Bioactive Bead-Mediated Transformation of Plants with Large DNA Fragments

      • Naoki Wada, Joyce A. Cartagena, Naruemon Khemkladngoen, Kiichi Fukui
      Pages 91-106

  8. Transformation Technology: Cereals and Other Monocots

    1. Front Matter

      Pages 107-107
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  9. TRANSFORMATION TECHNOLOGY – CEREALS AND OTHER MONOCOTS

    1. Agrobacterium-Mediated Transformation of Sorghum bicolor Using Immature Embryos

      • Songul Gurel, Ekrem Gurel, Tamara I. Miller, Peggy G. Lemaux
      Pages 109-122

    2. Split-Transgene Expression in Wheat

      • Mario Gils, Myroslava Rubtsova, Katja Kempe
      Pages 123-135

    3. Agrobacterium-Mediated Transformation of Brachypodium distachyon

      • Vera Thole, Philippe Vain
      Pages 137-149

    4. Transformation of Barley (Hordeum vulgare L.) by Agrobacterium tumefaciens Infection of In Vitro Cultured Ovules

      • Inger Bæksted Holme, Henrik Brinch-Pedersen, Mette Lange, Preben Bach Holm
      Pages 151-161

    5. Biolistic-Mediated Production of Transgenic Oil Palm

      • Ghulam Kadir Ahmad Parveez, Bohari Bahariah
      Pages 163-175

    6. Transformation of Oil Palm Using Agrobacterium tumefaciens

      • Abang Masli Dayang Izawati, Ghulam Kadir Ahmad Parveez, Mat Yunus Abdul Masani
      Pages 177-188
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Keywords

About this book

In 2010 the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, making it the most rapidly adopted technology in the history of modern agriculture. In Transgenic Plants: Methods and Protocols, Second Edition expert researchers in the field provide key techniques to investigate production and analysis of transgenic plants.  Focusing on selection and detection methods, transformation technology, gene targeting, silencing and directed mutation, metabolic engineering and pharming, the book encompasses protocols relating to major crops and model plants being used for genomic analysis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

Thorough and intuitive, Transgenic Plants: Methods and Protocols, Second Edition aids scientists in the continuous improvements being made for the production and analysis of transgenic plants.

Editors and Affiliations

  • School of Biological Sciences, University of Reading, Reading, Berkshire, United Kingdom

    Jim M. Dunwell

  • Dept. of Agricultural Botany, School of Biological Sciences, University of Reading, Reading, United Kingdom

    Andy C. Wetten

Bibliographic Information

  • Book Title: Transgenic Plants

  • Book Subtitle: Methods and Protocols

  • Editors: Jim M. Dunwell, Andy C. Wetten

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-558-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-557-2Published: 14 February 2012

  • Softcover ISBN: 978-1-4939-6078-1Published: 23 August 2016

  • eBook ISBN: 978-1-61779-558-9Published: 14 February 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 497

  • Number of Illustrations: 62 b/w illustrations, 30 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics, Transgenics

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