Adipose-Derived Stem Cells

1. Front Matter

   Pages i-xv

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2. Isolation Methods

   1. Front Matter

      Pages 1-1

      PDF

   2. Isolation and Culture of Rhesus Adipose-Derived Stem Cells

      • Christine Gagliardi, Bruce A. Bunnell

      Pages 3-16

   3. Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates

      • Gang Yu, Z. Elizabeth Floyd, Xiying Wu, Yuan-Di C. Halvorsen, Jeffrey M. Gimble

      Pages 17-27

   4. Isolation of Murine Adipose-Derived Stem Cells

      • Gang Yu, Xiying Wu, Gail Kilroy, Yuan-Di C. Halvorsen, Jeffrey M. Gimble, Z. Elizabeth Floyd

      Pages 29-36

   5. In Vitro Adult Rat Adipose Tissue-Derived Stromal Cell Isolation and Differentiation

      • Mandi J. Lopez, Nakia D. Spencer

      Pages 37-46

   6. In Vitro Adult Canine Adipose Tissue-Derived Stromal Cell Growth Characteristics

      • Nakia D. Spencer, Mandi J. Lopez

      Pages 47-60

   7. Adipogenic Differentiation of Adult Equine Mesenchymal Stromal Cells

      • Martin A. Vidal, Mandi J. Lopez

      Pages 61-75

   8. Isolation and Culture of Porcine Adipose Tissue-Derived Somatic Stem Cells

      • Kellie J. Williams, Robert A. Godke, Kenneth R. Bondioli

      Pages 77-86

   9. Automated Isolation and Processing of Adipose-Derived Stem and Regenerative Cells

      • Kevin C. Hicok, Marc H. Hedrick

      Pages 87-105

3. Phenotyping Methods

   1. Front Matter

      Pages 107-107

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   2. Methods for the Purification and Characterization of Human Adipose-Derived Stem Cells

      • Stan Gronthos, Andrew C. W. Zannettino

      Pages 109-120

   3. Characterization of Human Adipose-Derived Stem Cells Using Flow Cytometry

      • H. Alan Tucker, Bruce A. Bunnell

      Pages 121-131

   4. Evaluation of Cellular and Humoral Immune Responses to Allogeneic Adipose-Derived Stem/Stromal Cells

      • Kevin R. McIntosh

      Pages 133-150

   5. Adipose-Derived Stromal Cells: Cytokine Expression and Immune Cell Contaminants

      • Pauline Decaunes, David Estève, Alexia Zakaroff-Girard, Coralie Sengenès, Jean Galitzky, Anne Bouloumié

      Pages 151-161

   6. Gel-Based and Gel-Free Proteomic Technologies

      • Peter Scherp, Ginger Ku, Liana Coleman, Indu Kheterpal

      Pages 163-190

4. Differentiation Methods: In Vitro and In Vivo Applications

   1. Front Matter

      Pages 191-191

      PDF

   2. Adipogenic Differentiation of Adipose-Derived Stem Cells

      • Gang Yu, Z. Elizabeth Floyd, Xiying Wu, Teddi Hebert, Yuan-Di C. Halvorsen, Benjamin M. Buehrer et al.

      Pages 193-200

   3. Three-Dimensional Culture Systems to Induce Chondrogenesis of Adipose-Derived Stem Cells

      • Bradley T. Estes, Farshid Guilak

      Pages 201-217

   4. Neural Differentiation of Human Adipose Tissue-Derived Stem Cells

      • Ji Min Yu, Bruce A. Bunnell, Soo-Kyung Kang

      Pages 219-231

During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular Biology™ series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.

• Bioengineering
• Differentiation
• Human ASCs
• Isolation
• Phenotyping
• Regenerative medicine
• Stromal cells
• Tissue engineering

From the reviews:

“This book is pleasing the reader … . Clear enough, the statistics is impressive … . I am quite sure that the reader, after reading this unique and invaluable book, will easily share my idea that the ASC acronym, stending for Adipose-derived Stem/Stromal Cells and describing the collagenase-digested and culture-expanded adipose cell populations, will for sure appear more and more in the ISI statistics.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)

• , Stem Cell Biology, Pennington Biomedical Research Center, Baton Rouge, USA

  Jeffrey M. Gimble

• School of Medicine, Department of Pharmacology, Tulane University, New Orleans, USA

  Bruce A. Bunnell

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