Overview
- Comprised of both overview articles and detailed protocols for core proteomics techniques
- Covers the hot topics in proteomics research, such as relative and absolute protein quantification, organellar and membrane studies as well as PTM and protein interaction analysis
- Contains method descriptions for both beginners and advanced proteomics researchers on electrophoresis, chromatography, and mass spectrometry
- Provides guidance on proteomics methods from sample preparation to bioinformatical data interpretation written by experts in the respective fields
Part of the book series: Methods in Molecular Biology (MIMB, volume 564)
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Table of contents (23 protocols)
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Front Matter
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Introduction
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Front Matter
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Electrophorestic Separations
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Front Matter
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Mass Spectrometry and Tandem Mass Spectrometry Applications
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Front Matter
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Interpretation of Mass Spectrometry Data
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Front Matter
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Keywords
About this book
Since the development of the concept of “proteomics”, great progress has granted deeper insight into the cellular network, but the enormous range of protein abundance, dynamics, and interactions leaves many further challenges in the field. In Proteomics: Methods and Protocols, experts in proteomic research have contributed detailed techniques, which present an overview of the contemporary challenges and possibilities in the various areas of proteomics, as well as cutting-edge protocols that provide concrete examples for successful proteome analysis. Written in the clear and concise Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easy-to-use, Proteomics: Methods and Protocols will serve as a valuable reference book for laboratories around the world and raise interest in the complex and vital field of proteomics research.
Bibliographic Information
Book Title: Proteomics
Book Subtitle: Methods and Protocols
Editors: Jörg Reinders, Albert Sickmann
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-157-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60761-156-1Published: 29 June 2009
Softcover ISBN: 978-1-62703-831-7Published: 27 November 2014
eBook ISBN: 978-1-60761-157-8Published: 20 June 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 441
Topics: Protein Science, Biochemistry, general, Proteomics