Overview
- Covers key techniques for zebrafish mutagenesis, transgenesis, and gene expression analysis and imaging
- Contains practical approaches to chemical and insertional mutagenesis, including frozen storage of zebrafish sperm
- Includes a range of transgenesis protocols (building transgenes from bacterial artificial chromosomes, transposon- and meganuclease-assisted transgenesis)
- Describes imaging techniques ranging from organ-specific examples to whole embryo in toto time-lapse imaging
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 546)
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Table of contents (19 protocols)
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Mutagenesis and Mutants
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Transgenesis
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Tissue-Specific Manipulations
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Analyzing Gene Expression
Keywords
About this book
In the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically, due in part to the ease of breeding and raising them, their genetic tractability, embryonic accessibility, and their imaging potential. In Zebrafish: Methods and Protocols, expert researchers provide a collection of experimental techniques for the genetic modification, manipulation, and in vivo live imaging of zebrafish. This volume collects sophisticated and practical techniques, including several not yet widely known, from laboratories around the world. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Zebrafish: Methods and Protocols seeks to aid and inspire scientists utilizing this versatile animal model in their research.
Editors and Affiliations
Bibliographic Information
Book Title: Zebrafish
Book Subtitle: Methods and Protocols
Editors: Graham J. Lieschke, Andrew C. Oates, Koichi Kawakami
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-977-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60327-976-5Published: 15 May 2009
Softcover ISBN: 978-1-62703-899-7Published: 27 November 2014
eBook ISBN: 978-1-60327-977-2Published: 20 April 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 335
Topics: Fish & Wildlife Biology & Management, Biochemistry, general, Animal Models