Skip to main content
SpringerLink
Log in
Book cover
  • Book
  • © 1996

The Protein Protocols Handbook

Editors:

  1. John M. Walker

    1. University of Hertfordshire, Hatfield, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Springer Protocols Handbooks (SPH)

Sections

Buy it now

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (144 protocols)

  1. Front Matter

    Pages i-xviii
    PDF

  2. Quantitation of Proteins

    1. Front Matter

      Pages 1-1
      PDF

    2. Protein Determination by UV Absorption

      • Alastair Aitken, Michèle Learmonth
      Pages 3-6

    3. The Lowry Method for Protein Quantitation

      • Jakob H. Waterborg, Harry R. Matthews
      Pages 7-9

    5. The Bradford Method for Protein Quantitation

      • Nicholas J. Kruger
      Pages 15-20

    6. Ultrafast Protein Determinations Using Microwave Enhancement

      • Robert E. Akins, Rocky S. Tuan
      Pages 21-28

    7. Quantitation of Tryptophan in Proteins

      • Alastair Aitken, Michèle Learmonth
      Pages 29-31

    8. Protein Quantitation Using Flow Cytometry

      • F. Andrew Ray, Thomas D. Friedrich, Judith Laffin, John M. Lehman
      Pages 33-38

    9. Copper Iodide Staining of Proteins on Solid Phases

      • Douglas D. Root, Kuan Wang
      Pages 39-43

  3. Electrophoresis of Proteins and Peptides and Detection in Gels

    1. Front Matter

      Pages 49-49
      PDF

    7. Acid-Urea-Triton Polyacrylamide Gels for Histones

      • Jakob H. Waterborg
      Pages 91-100
Back to top

About this book

In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.
Back to top

Keywords

Back to top

Reviews

The Protein Protocols Handbook has arrived at the right place at the right time.-Trends in Cell Biology
Back to top

Editors and Affiliations

  • University of Hertfordshire, Hatfield, UK

    John M. Walker

Back to top

Bibliographic Information

  • Book Title: The Protein Protocols Handbook

  • Editors: John M. Walker

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-1-60327-259-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media LLC 1996

  • eBook ISBN: 978-1-60327-259-9Published: 12 February 2008

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XLIV, 809

  • Topics: Biochemistry, general

Back to top

Publish with us

Policies and ethics

Back to top
Access via your institution

Buy it now

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

Search

Navigation