Stem Cell Assays

1. Front Matter

   Pages i-xiv

   PDF

2. Derivation of Human Embryonic Stem Cells in Xeno-Free Conditions

   • Mohan C. Vemuri, Tim Schimmel, Pere Colls, Santiago Munne, Jacques Cohen

   Pages 1-10

3. Feeder-Layer Free Culture System for Human Embryonic Stem Cells

   • Michal Amit

   Pages 11-20

4. Digital Imaging of Stem Cells by Electron Microscopy

   • A. Henry Sathananthan, Stefania A. Nottola

   Pages 21-41

5. A Controlled-Cooling Protocol for Cryopreservation of Human and Non-Human Primate Embryonic Stem Cells

   • Carol B. Ware, Szczepan W. Baran

   Pages 43-49

6. Cell Surface Markers in Human Embryonic Stem Cells

   • Raj R. Rao, Alison Venable Johnson, Steven L. Stice

   Pages 51-61

7. Generation of a Monoclonal Antibody Library Against Human Embryonic Stem Cells

   • Micha Drukker, Christina Muscat, Irving L. Weissman

   Pages 63-81

8. Analytical Methods for Cancer Stem Cells

   • Vinagolu K. Rajasekhar

   Pages 83-95

9. Micro RNA Profiling

   • Uma Lakshmipathy, Brad Love, Christopher Adams, Bhaskar Thyagarajan, Jonathan D. Chesnut

   Pages 97-114

10. Gene Transfer Via Nucleofection Into Adult and Embryonic Stem Cells

    • Uma Lakshmipathy, Shannon Buckley, Catherine Verfaillie

    Pages 115-126

11. RNAi Knockdown of Transcription Factor Pu.1 in the Differentiation of Mouse Embryonic Stem Cells

    • Gang-Ming Zou, Meredith A. Thompson, Mervin C. Yoder

    Pages 127-136

12. StemBase

    • Christopher J. Porter, Gareth A. Palidwor, Reatha Sandie, Paul M. Krzyzanowski, Enrique M. Muro, Carolina Perez-Iratxeta et al.

    Pages 137-148

13. Isolation of Stem Cells from Human Umbilical Cord Blood

    • Nishanth P. Reddy, Mohan C. Vemuri, Reddanna Pallu

    Pages 149-163

14. Ex Vivo Expansion of Hematopoietic Stem Cells from Human Cord Blood in Serum-Free Conditions

    • Chao-Ling Yao, Shiaw-Min Hwang

    Pages 165-175

15. Hematopoietic Colony-Forming Cell Assays

    • Carla Pereira, Emer Clarke, Jackie Damen

    Pages 177-208

16. Assays for Alloreactive Responses by PCR

    • Patrick Stordeur

    Pages 209-224

17. Immune Properties of Mesenchymal Stem Cells

    • Panagiota A. Sotiropoulou, Michael Papamichail

    Pages 225-243

18. Clinical Grade Expansion of Human Bone Marrow Mesenchymal Stem Cells

    • Panagiota A. Sotiropoulou, Sonia A. Perez, Michael Papamichail

    Pages 245-263

19. Adenoviral Transduction of Mesenchymal Stem Cells

    • Pablo Bosch, Steven L. Stice

    Pages 265-274

20. Directed Differentiation of Human Embryonic Stem Cells to Dendritic Cells

    • Maxim A. Vodyanik, Igor I. Slukvin

    Pages 275-293

Stem cells and regenerative medicine is a fast emerging field with rapid strides of progress and focus on human health. Successful utilization of stem cells in regenerative medicine depends on two important features. One, stem cells can grow and divide indefinitely, and two, stem cells can differentiate into specialized cell types that make up tissues such as pancreas, heart, liver, blood, and others. Utilizing the property of differentiation, stem cells can be engineered to produce cell types to replace wornout cells and to regenerate damaged tissue. The scope for improving healthcare using stem cell therapies is thrilling, but considerable technical challenges and methodological constraints need to be addressed. There is a great need for isolation and propagation of “transplant-ready” or “clinical-grade” stem cells and screening methods, well before they are used in therapies. Recent scientific advances have contributed enormously to the development of new methods pertaining to both embryonic and adult stem cell isolation, characterization, expansion in undifferentiated state, customized differentiation, efficient stem cell transduction for gene therapy, quick monitoring of rejection, through polymerase chain reaction approaches following transplantation, and more. The scientific progress that makes stem cell biology so exciting also threatens to suffocate researchers with an avalanche of information. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, the book Stem Cell Assays aims to present scientific advances in stem cell methods for a wider use by novice and expert scientists, through the Methods in Molecular Biology series.

• PCR
• RNA
• antibody
• cancer
• cell
• electron microscopy
• gene expression
• gene transfer
• genes
• microscopy
• molecular biology
• stem cell
• stem cells
• tissue
• transcription

From the reviews:

"The present volume ‘Stem Cell Assays’ … include very detailed experimental sections, which are essentially raw lab protocols. … All protocols are explained in full detail … and should be very useful for researchers novel to this field. In addition, for more experienced scientists it may be very useful to compare their own protocols with those provided in this volume … . this book will be of great value for experimental scientists that wish to introduce novel technology into their labs." (Gerald de Haan, Molecular Biotechnology, May, 2008)

• Stem Cells, Reprogenetics, West Orange

  Mohan C. Vemuri

• Invitrogen Corporation, Grand Island

  Mohan C. Vemuri

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