PCR Primer Design

Editors:

ISBN: 978-1-58829-725-9 (Print) 978-1-59745-528-2 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xiii

  2. Basic Principles and Software for PCR Primer Design

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-33

      Physical Principles and Visual-OMP Software for Optimal PCR Design

    3. No Access

      Book Protocol

      Pages 35-59

      OLIGO 7 Primer Analysis Software

    4. No Access

      Book Protocol

      Pages 61-74

      Selection for 3′-End Triplets for Polymerase Chain Reaction Primers

    5. No Access

      Book Protocol

      Pages 75-91

      The Reference Point Method in Primer Design

    6. No Access

      Book Protocol

      Pages 93-103

      PCR Primer Design Using Statistical Modeling

    7. No Access

      Book Protocol

      Pages 105-137

      Developing a Statistical Model for Primer Design

  3. Genome-Scale PCR Primer Design

    1. Front Matter

      Pages 139-139

    2. No Access

      Book Protocol

      Pages 141-157

      GST-PRIME

    3. No Access

      Book Protocol

      Pages 159-175

      Genome-Scale Probe and Primer Design with PRIMEGENS

  4. Repeat Masking for PCR Primer Design

    1. Front Matter

      Pages 177-177

    2. Repeat Masking for PCR Primer Design

      1. No Access

        Book Protocol

        Pages 178-199

        SNPbox

      2. No Access

        Book Protocol

        Pages 201-217

        Fast Masking of Repeated Primer Binding Sites in Eukaryotic Genomes

  5. Multiplex PCR Primer Design

    1. Front Matter

      Pages 219-219

    2. Multiplex PCR Primer Design

      1. No Access

        Book Protocol

        Pages 220-244

        Degenerate Primer Design

      2. No Access

        Book Protocol

        Pages 245-267

        An Iterative Method for Selecting Degenerate Multiplex PCR Primers

      3. No Access

        Book Protocol

        Pages 269-285

        Primer Design for Multiplexed Genotyping

      4. No Access

        Book Protocol

        Pages 287-303

        MultiPLX

      5. No Access

        Book Protocol

        Pages 305-314

        MultiPrimer

  6. Allele-specific PCR

    1. Front Matter

      Pages 315-315

    2. Allele-specific PCR

      1. No Access

        Book Protocol

        Pages 316-327

        Modified Oligonucleotides as Tools for Allele-Specific Amplification

      2. No Access

        Book Protocol

        Pages 329-345

        AlleleID

  7. Long PCR Primer Design

    1. Front Matter

      Pages 347-347

    2. Long PCR Primer Design

      1. No Access

        Book Protocol

        Pages 348-368

        Designing Primers for Whole Genome PCR Scanning Using the Software Package GenoFrag

  8. DNA Methylation Mapping

    1. Front Matter

      Pages 369-369

    2. DNA Methylation Mapping

      1. No Access

        Book Protocol

        Pages 370-383

        Designing PCR Primer for DNA Methylation Mapping

      2. No Access

        Book Protocol

        Pages 385-402

        BiSearch

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