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Lipid Rafts

  • Book
  • © 2007

Overview

Editors:
  1. Thomas J. McIntosh

    1. Department of Cell Biology, Duke University Medical Center, Durham

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  • In-depth examination of sophisticated techniques used to study intact cell membranes
  • Proven, detailed biochemical, biophysical, and computational methods and protocols

Part of the book series: Methods in Molecular Biology (MIMB, volume 398)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Overview of Membrane Rafts

    • Thomas J. McIntosh
    Pages 1-7

  3. Analysis of Raft Affinity of Membrane Proteins by Detergent-Insolubility

    • Deborah A. Brown
    Pages 9-20

  4. Nondetergent Isolation of Rafts

    • Mehul B. Shah, Pravin B. Sehgal
    Pages 21-28

  5. Detecting Ordered Domain Formation (Lipid Rafts) in Model Membranes Using Tempo

    • Omar Bakht, Erwin London
    Pages 29-40

  6. Using Monomolecular Films to Characterize Lipid Lateral Interactions

    • Rhoderick E. Brown, Howard L. Brockman
    Pages 41-58

  7. Electro-Formation and Fluorescence Microscopy of Giant Vesicles With Coexisting Liquid Phases

    • Sarah L. Veatch
    Pages 59-72

  8. Fluorescence Correlation Spectroscopy

    • Kirsten Bacia, Petra Schwille
    Pages 73-84

  9. Multiphoton Laser-Scanning Microscopy and Spatial Analysis of Dehydroergosterol Distributions on Plasma Membrane of Living Cells

    • Avery L. McIntosh, Barbara P. Atshaves, Huan Huang, Adalberto M. Gallegos, Ann B. Kier, Friedhelm Schroeder et al.
    Pages 85-105

  10. NMR Detection of Lipid Domains

    • Ivan V. Polozov, Klaus Gawrisch
    Pages 107-126

  11. Lateral Diffusion Coefficients of Raft Lipids From Pulsed Field Gradient NMR

    • Greger Orädd, Göran Lindblom
    Pages 127-142

  12. Saturation-Recovery Electron Paramagnetic Resonance Discrimination by Oxygen Transport (DOT) Method for Characterizing Membrane Domains

    • Witold K. Subczynski, Justyna Widomska, Anna Wisniewska, Akihiro Kusumi
    Pages 143-157

  13. Plasmon-Waveguide Resonance Spectroscopy Studies of Lateral Segregation in Solid-Supported Proteolipid Bilayers

    • Zdzislaw Salamon, Savitha Devanathan, Gordon Tollin
    Pages 159-178

  14. Fluorescence Recovery After Photobleaching Studies of Lipid Rafts

    • Anne K. Kenworthy
    Pages 179-192

  15. Single-Molecule Tracking

    • Marija Vrljic, Stefanie Y. Nishimura, W. E. Moerner
    Pages 193-219

  16. X-Ray Diffraction to Determine the Thickness of Raft and Nonraft Bilayers

    • Thomas J. McIntosh
    Pages 221-230

  17. Small-Angle Neutron Scattering to Detect Rafts and Lipid Domains

    • Jeremy Pencer, Thalia T. Mills, Norbert Kucerka, Mu-Ping Nieh, John Katsaras
    Pages 231-244

  18. Exploring Membrane Domains Using Native Membrane Sheets and Transmission Electron Microscopy

    • Bridget S. Wilson, Janet R. Pfeiffer, Mary Ann Raymond-Stintz, Diane Lidke, Nicholas Andrews, Jun Zhang et al.
    Pages 245-261

  19. Atomic Force Microscopy of Lipid Domains in Supported Model Membranes

    • Alan R. Burns
    Pages 263-282

  20. Atomistic and Coarse-Grained Computer Simulations of Raft-Like Lipid Mixtures

    • Sagar A. Pandit, H. Larry Scott
    Pages 283-302
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Keywords

About this book

In the past several years significant attention has been given to the analysis of the properties and functions of lateral microdomains (rafts) in biological membranes. As described in the overview chapter of this book, as well as in the introductions to many of the other chapters, there are many fundamental un- swered questions concerning the composition, structure, dynamics, and even the very existence of these membrane rafts. Therefore, a variety of sophisticated techniques have been used to study intact cell membranes, as well as model s- tems composed of specific lipids and proteins thought to be in rafts. The analyses of biological membranes have provided detailed data on intact microdomains, and the complementary studies of model systems have given critical insights on the roles of specific lipid-lipid and lipid-protein interactions in raft formation and function. The chapters in this book provide detailed information on modern methods that are currently being employed to study lipid rafts.

Editors and Affiliations

  • Department of Cell Biology, Duke University Medical Center, Durham

    Thomas J. McIntosh

Bibliographic Information

  • Book Title: Lipid Rafts

  • Editors: Thomas J. McIntosh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-513-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-729-7Published: 06 July 2007

  • Softcover ISBN: 978-1-61737-744-0Published: 19 November 2010

  • eBook ISBN: 978-1-59745-513-8Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 326

  • Number of Illustrations: 104 b/w illustrations, 8 illustrations in colour

  • Topics: Biochemistry, general, Biological Microscopy, Cell Biology

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