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Baculovirus and Insect Cell Expression Protocols

  • Book
  • © 2007

Overview

Editors:
  1. David W. Murhammer

    1. Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City

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  • Includes useful tips for baculovirus research and detailed procedures for quantifying cell metabolic behavior
  • Lists all available insect cell lines and provides methods for isolating new cell lines
  • Numerous methods and protocols to inspire new techniques and applications for baculovirus and insect cell culture

Part of the book series: Methods in Molecular Biology (MIMB, volume 388)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Useful Tips, Widely Used Techniques, and Quantifying Cell Metabolic Behavior

      • David W. Murhammer
      Pages 3-22

  3. Baculovirus Molecular Biology/Development of Recombinant Baculoviruses

    1. Front Matter

      Pages 23-23
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    2. Introduction to Baculovirus Molecular Biology

      • Barbara J. Kelly, Linda A. King, Robert D. Possee
      Pages 25-53

    3. Baculovirus Transfer Vectors

      • Robert D. Possee, Linda A. King
      Pages 55-75

    4. Recombinant Baculovirus Isolation

      • Linda A. King, Richard Hitchman, Robert D. Possee
      Pages 77-93

    5. Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System

      • James A. Fornwald, Quinn Lu, Dayuan Wang, Robert S. Ames
      Pages 95-114

  5. Protein Production with Recombinant Baculoviruses

    1. Front Matter

      Pages 209-209
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    2. Small-Scale Protein Production With the Baculovirus Expression Vector System

      • Steve Harwood
      Pages 211-223

    3. Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System

      • Cynthia B. Elias, Barbara Jardin, Amine Kamen
      Pages 225-245

    4. Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors

      • Sue H. Kadwell, Philip I. Hardwicke
      Pages 247-266

    5. Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae

      • Yi Liu, Nathan De Carolis, Nikolai van Beek
      Pages 267-279

    6. Virus-like Particle and Viral Vector Production Using the Baculovirus Expression Vector System/Insect Cell System

      • Marc G. Aucoin, Danielle Jacob, Parminder Singh Chahal, Jamal Meghrous, Alice Bernier, Amine A. Kamen
      Pages 281-296

  6. Recombinant Protein Production with Transformed Insect Cells

    1. Front Matter

      Pages 297-297
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Keywords

About this book

Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology™ series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.

Editors and Affiliations

  • Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City

    David W. Murhammer

Bibliographic Information

  • Book Title: Baculovirus and Insect Cell Expression Protocols

  • Editors: David W. Murhammer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-457-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-537-8Published: 06 July 2007

  • Softcover ISBN: 978-1-61737-629-0Published: 19 November 2010

  • eBook ISBN: 978-1-59745-457-5Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 470

  • Topics: Microbiology, Biochemistry, general, Plant Biochemistry, Cell Biology, Plant Sciences

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