Overview
- Serves as a detailed step-by-step laboratory manual covering the most up-to-date, widely used protocols to study the biology of the cyclooxygenase enzymes
- Includes chapters written by some of the pioneers in the cyclooxygenase field
- Acts as a reference that no cyclooxygenase or prostanoid research laboratory should be without
- An indispensable tool for pharmaceutical companies engaged in the discovery and development of cyclooxygenase inhibitors
- Describes molecular and biochemical techniques used to study the purification, cloning, expression, and activity of the cyclooxygenase enzymes
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 644)
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Table of contents (18 protocols)
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Front Matter
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In Vitro Protocols for Studying Expression and Activity of Cyclooxygenase Enzymes and Characterisation of Their Inhibition Patterns
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Front Matter
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Extraction and Measurement of Prostanoids and Isoprostanes
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Front Matter
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In Vivo Models to Study Involvement of Cyclooxygenase Products in Health and Disease
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Front Matter
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Keywords
About this book
Since the discovery of the pharmacological and toxicological importance of inhibiting the cyclooxygenase (COX) enzymes by non-steroidal anti-inflammatory drugs (NSAIDs), much research has gone into the development of methods to study the biological functions of COX-1 and COX-2. In Cyclooxygenases: Methods and Protocols, experts and pioneers in the field present the most up-to-date in vitro and in vivo techniques routinely used in COX research. The volume delves into essential topics such as the purification, cloning, and expression of COX enzymes as well as in vitro assays aimed at determining the inhibitory potency of drugs on COX-1 and COX-2 activities, with chapters describing protocols used for the extraction and measurement of the prostanoids. This volume also describes in vivo disease models used to study the roles of COX-1 and COX-2 in gastrointestinal injury, inflammation, and pain. As a book in the highly successful Methods in Molecular Biologyâ„¢ series, the protocols chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Cyclooxygenases: Methods and Protocols serves as an indispensable tool for all scientists seeking the treatment of inflammation, pain, fever, and other harmful conditions.
Editors and Affiliations
Bibliographic Information
Book Title: Cyclooxygenases
Book Subtitle: Methods and Protocols
Editors: Samir S. Ayoub, Roderick J. Flower, Michael P. Seed
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-364-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2010
Hardcover ISBN: 978-1-58829-953-6Published: 07 May 2010
Softcover ISBN: 978-1-4939-5638-8Published: 23 August 2016
eBook ISBN: 978-1-59745-364-6Published: 20 July 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 218