Overview
- Step-by-step protocols for the most commonly used models of acute and chronic ethanol exposure
- Detailed procedures for cell culture experiments with ethanol
- Comprehensive methods for studies of fetal alcohol syndrome (FAS) including Zebrafish, avian embryo and rodent models
- Up-to-date proteomics and micro-array protocols
- Specific protocols of interest to neuroscientists, immunologists, physiologists, cell biologists and biochemists
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 447)
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Table of contents (26 protocols)
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Animal Models of Acute and Chronic Ethanol Exposure
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Animal Models of Ethanol Exposure During Development
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Cell Culture Approaches to Studying Ethanol Exposure
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Regulation of Specific Organ Systems in Response to Ethanol
Keywords
About this book
Many unexplored complex cellular and organismal adaptations occur in response to the stress of alcohol exposure, and its contribution to the development of chronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population. In Alcohol: Methods and Protocols, the pleiotropic effects of ethanol in animal and cell culture models are rigorously examined through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics.
Editors and Affiliations
Bibliographic Information
Book Title: Alcohol
Book Subtitle: Methods and Protocols
Editors: Laura E. Nagy
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-242-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-906-2Published: 07 March 2008
Softcover ISBN: 978-1-61737-837-9Published: 19 November 2010
eBook ISBN: 978-1-59745-242-7Published: 15 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XX, 420
Number of Illustrations: 72 b/w illustrations, 2 illustrations in colour
Topics: Pharmacology/Toxicology, Human Physiology, Pathology, Molecular Medicine, Cell Biology