Book Volume 1566 2017

Thermogenic Fat

Methods and Protocols

Editors:

ISBN: 978-1-4939-6819-0 (Print) 978-1-4939-6820-6 (Online)

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi

  2. Isolation and Culture Methods

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-8

      Isolation, Primary Culture, and Differentiation of Preadipocytes from Mouse Brown Adipose Tissue

    3. No Access

      Protocol

      Pages 9-16

      Isolation of Mouse Stromal Vascular Cells for Monolayer Culture

    4. No Access

      Protocol

      Pages 17-24

      Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells

    5. No Access

      Protocol

      Pages 25-36

      Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes

    6. No Access

      Protocol

      Pages 37-48

      Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells

    7. No Access

      Protocol

      Pages 49-59

      Isolation of Immune Cells from Adipose Tissue for Flow Cytometry

    8. No Access

      Protocol

      Pages 61-76

      Differentiation and Metabolic Interrogation of Human Adipocytes

    9. No Access

      Protocol

      Pages 77-85

      Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines

  3. Functional Analysis, Bioengineering, and Other Applications

    1. Front Matter

      Pages 87-87

    2. No Access

      Protocol

      Pages 89-98

      Gene Expression and Histological Analysis of Activated Brown Adipocytes in Adipose Tissue

    3. No Access

      Protocol

      Pages 99-107

      Genetic Mouse Models: The Powerful Tools to Study Fat Tissues

    4. No Access

      Protocol

      Pages 109-124

      Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function

    5. No Access

      Protocol

      Pages 125-134

      Bioenergetic Analyses in Adipose Tissue

    6. No Access

      Protocol

      Pages 135-143

      Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry

    7. No Access

      Protocol

      Pages 145-150

      Isolation and Patch-Clamp of Primary Adipocytes

    8. No Access

      Protocol

      Pages 151-158

      In Vitro Approaches to Model and Study Communication Between Adipose Tissue and the Liver

    9. No Access

      Protocol

      Pages 159-176

      Identification and Quantification of Human Brown Adipose Tissue

    10. No Access

      Protocol

      Pages 177-183

      Designing 3-D Adipospheres for Quantitative Metabolic Study

    11. No Access

      Protocol

      Pages 185-201

      Culture and Sampling of Primary Adipose Tissue in Practical Microfluidic Systems

    12. No Access

      Protocol

      Pages 203-212

      Using Thermogenic Beige Cells to Identify Biologically Active Small Molecules and Peptides

  4. Back Matter

    Pages 213-214