Book Volume 1554 2017

Signal Transduction Immunohistochemistry

Methods and Protocols

Editors:

ISBN: 978-1-4939-6757-5 (Print) 978-1-4939-6759-9 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-x

  2. No Access

    Protocol

    Pages 1-40

    Phosphosite-Specific Antibodies: A Brief Update on Generation and Applications

  3. No Access

    Protocol

    Pages 41-59

    Antibodies That Work Again and Again and Again

  4. No Access

    Protocol

    Pages 61-73

    Validating Antibody Specificities for Immunohistochemistry by Protein Blotting Methods

  5. No Access

    Protocol

    Pages 75-106

    Acquisition and Post-Processing of Immunohistochemical Images

  6. No Access

    Protocol

    Pages 107-125

    Using Digital Quantification of Stained Tissue Microarrays as a Medium-Throughput, Quantitative Method for Measuring the Kinetics of Signal Transduction

  7. No Access

    Protocol

    Pages 127-141

    EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis

  8. No Access

    Protocol

    Pages 143-153

    Preservation of Specific Protein Signaling States Using Heat Based Stabilizor System

  9. No Access

    Protocol

    Pages 155-160

    Hapten–Anti-Hapten Technique for Two-Color IHC Detection of Phosphorylated EGFR and H2AX Using Primary Antibodies Raised in the Same Host Species

  10. No Access

    Protocol

    Pages 161-173

    Culture, Transfection, and Immunocytochemical Analysis of Primary Macrophages

  11. No Access

    Protocol

    Pages 175-184

    Phenotyping CD4+ hTh2 Cells by Flow Cytometry: Simultaneous Detection of Transcription Factors, Secreted Cytokines, and Surface Markers

  12. No Access

    Protocol

    Pages 185-191

    A Coupled Approach Utilizing Immunohistochemistry and Immunocytochemistry to Visualize Cellular Lipophagy

  13. No Access

    Protocol

    Pages 193-200

    Apoptotic Bodies: Selective Detection in Extracellular Vesicles

  14. No Access

    Protocol

    Pages 201-209

    Activation and Differentiation of Mesenchymal Stem Cells

  15. No Access

    Protocol

    Pages 211-218

    High-Sensitivity IHC Detection of Phosphorylated p27/Kip1 in Human Tissues Using Secondary Antibody Conjugated to Polymer-HRP

  16. No Access

    Protocol

    Pages 219-227

    Using Phospho-Peptides Immobilized on Magnetic Beads for Absorption Control in Immunohistochemistry

  17. No Access

    Protocol

    Pages 229-236

    Dual Detection of Nucleolytic and Proteolytic Markers of Lysosomal Cell Death: DNase II-Type Breaks and Cathepsin D

  18. No Access

    Protocol

    Pages 237-251

    Multiplex Immunohistochemistry for Mapping the Tumor Microenvironment

  19. No Access

    Protocol

    Pages 253-262

    Validation of Anti-Mouse PDL-1 Goat Polyclonal Antibody Staining with Mouse PDL-1 In Situ Hybridization on Adjacent Sections of Cell Pellets and Mouse Tumors

  20. No Access

    Protocol

    Pages 263-272

    Automated Double In Situ Detection of Mouse Lgr5 mRNA and Lysozyme Protein in Examining the Neighboring Cell Types of the Mouse Intestinal Crypt

  21. No Access

    Protocol

    Pages 273-283

    Automated High-Content Screening of γ-H2AX Expression in HeLa Cells

  22. Back Matter

    Pages 285-290