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Oral Biology

Molecular Techniques and Applications

  • Book
  • © 2017

Overview

Editors:
  1. Gregory J. Seymour

    1. University of Otago Fac. Dentistry, Dunedin, New Zealand

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    You can also search for this editor in PubMed   Google Scholar

  2. Mary P. Cullinan

    1. Department of Oral Sciences, University of Otago Faculty of Dentistry, Dunedin, New Zealand

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Nicholas C.K. Heng

    1. Sir John Walsh Research Institute, University of Otago Faculty of Dentistry, Dunedin, New Zealand

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1537)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Saliva and Other Oral Fluids

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Salivary Diagnostics Using Purified Nucleic Acids

      • Paul D. Slowey
      Pages 3-15

    3. RNA Sequencing Analysis of Salivary Extracellular RNA

      • Blanca Majem, Feng Li, Jie Sun, David T. W. Wong
      Pages 17-36

    4. Qualitative and Quantitative Proteome Analysis of Oral Fluids in Health and Periodontal Disease by Mass Spectrometry

      • Erdjan Salih
      Pages 37-60

    5. Antioxidant Micronutrients and Oxidative Stress Biomarkers

      • Iain L. C. Chapple, Helen R. Griffiths, Mike R. Milward, Martin R. Ling, Melissa M. Grant
      Pages 61-77

    6. NMR-Based Metabolomics of Oral Biofluids

      • Horst Joachim Schirra, Pauline J. Ford
      Pages 79-105

    7. Gene Therapy of Salivary Diseases

      • Bruce J. Baum, Sandra Afione, John A. Chiorini, Ana P. Cotrim, Corinne M. Goldsmith, Changyu Zheng
      Pages 107-123

  3. Molecular Biosciences

    1. Front Matter

      Pages 125-125
      Download chapter PDF

    2. The Oral Microbiota in Health and Disease: An Overview of Molecular Findings

      • José F. Siqueira Jr., Isabela N. Rôças
      Pages 127-138

    3. Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)

      • José F. Siqueira Jr., Mitsuo Sakamoto, Alexandre S. Rosado
      Pages 139-152

    4. Analysis of 16S rRNA Gene Amplicon Sequences Using the QIIME Software Package

      • Blair Lawley, Gerald W. Tannock
      Pages 153-163

    5. Adhesion of Yeast and Bacteria to Oral Surfaces

      • Richard D. Cannon, Karl M. Lyons, Kenneth Chong, Kathryn Newsham-West, Kyoko Niimi, Ann R. Holmes
      Pages 165-190

    6. Quantitative Analysis of Periodontal Pathogens Using Real-Time Polymerase Chain Reaction (PCR)

      • Mª José Marin, Elena Figuero, David Herrera, Mariano Sanz
      Pages 191-202

    7. Methods to Study Antagonistic Activities Among Oral Bacteria

      • Fengxia Qi, Jens Kreth
      Pages 203-218

    8. Natural Transformation of Oral Streptococci by Use of Synthetic Pheromones

      • Gabriela Salvadori, Roger Junges, Rabia Khan, Heidi A. Åmdal, Donald A. Morrison, Fernanda C. Petersen
      Pages 219-232

    9. Markerless Genome Editing in Competent Streptococci

      • Roger Junges, Rabia Khan, Yanina Tovpeko, Heidi A. Åmdal, Fernanda C. Petersen, Donald A. Morrison
      Pages 233-247

    10. Tools and Strategies for Analysis of Genome-Wide and Gene-Specific DNA Methylation Patterns

      • Aniruddha Chatterjee, Euan J. Rodger, Ian M. Morison, Michael R. Eccles, Peter A. Stockwell
      Pages 249-277

    11. Generating Multiple Base-Resolution DNA Methylomes Using Reduced Representation Bisulfite Sequencing

      • Aniruddha Chatterjee, Euan J. Rodger, Peter A. Stockwell, Gwenn Le Mée, Ian M. Morison
      Pages 279-298

    12. A Protocol for the Determination of the Methylation Status of Gingival Tissue DNA at Specific CpG Islands

      • Trudy J. Milne
      Pages 299-306
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Keywords

About this book

This second edition volume presents a selection of cellular and molecular techniques that can be adapted to cover a range of applications and diseases. The book is divided into three sections: saliva and oral diseases, molecular biosciences, and cell and tissues. The first section contains chapters that discuss proteomic analyses by mass spectrometry and NMR-based metabolomics that can be used to not only study saliva, but also to assess other oral fluids such as gingival crevicular fluid. The second section contains chapters that profile oral microbial communities, quantitative real-time PCR, and adhesion of yeast and bacteria to oral surfaces. The third section deals with a range of approaches that enable the behavior of cells and tissues in both health and disease to be analyzed at the molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and comprehensive, Oral Biology: Molecular Techniques and Applications, Second Edition is a useful resource for oral biologists at all levels (students, early career researchers, and experienced veterans), and it provides a ready reference to new techniques and approaches that can be used to answer numerous specific scientific questions that may lead to a deeper understanding and treatments of oral diseases.

Editors and Affiliations

  • University of Otago Fac. Dentistry, Dunedin, New Zealand

    Gregory J. Seymour

  • Department of Oral Sciences, University of Otago Faculty of Dentistry, Dunedin, New Zealand

    Mary P. Cullinan

  • Sir John Walsh Research Institute, University of Otago Faculty of Dentistry, Dunedin, New Zealand

    Nicholas C.K. Heng

Bibliographic Information

  • Book Title: Oral Biology

  • Book Subtitle: Molecular Techniques and Applications

  • Editors: Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6685-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6683-7Published: 07 December 2016

  • Softcover ISBN: 978-1-4939-8272-1Published: 07 July 2018

  • eBook ISBN: 978-1-4939-6685-1Published: 05 December 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 503

  • Number of Illustrations: 38 b/w illustrations, 54 illustrations in colour

  • Topics: Molecular Medicine

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