Book Volume 1535 2017

Bacterial Pathogenesis

Methods and Protocols

Editors:

ISBN: 978-1-4939-6671-4 (Print) 978-1-4939-6673-8 (Online)

Table of contents (23 protocols)

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  1. Front Matter

    Pages i-xi

  2. Identification and Characterization of Bacterial Effector Molecules

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-15

      Protein-Based Strategies to Identify and Isolate Bacterial Virulence Factors

    3. No Access

      Protocol

      Pages 17-24

      Analysis of Bacterial Surface Interactions with Mass Spectrometry-Based Proteomics

    4. No Access

      Protocol

      Pages 25-41

      Differential Radial Capillary Action of Ligand Assay (DRaCALA) for High-Throughput Detection of Protein–Metabolite Interactions in Bacteria

    5. No Access

      Protocol

      Pages 43-61

      Identifying Bacterial Immune Evasion Proteins Using Phage Display

  3. Structural Biology of Bacterial–Host Interactions

    1. Front Matter

      Pages 63-63

    2. No Access

      Protocol

      Pages 65-75

      Competition for Iron Between Host and Pathogen: A Structural Case Study on Helicobacter pylori

    3. No Access

      Protocol

      Pages 77-93

      Common Challenges in Studying the Structure and Function of Bacterial Proteins: Case Studies from Helicobacter pylori

  4. Genetics and Phylogenetics of Bacterial Pathogens

    1. Front Matter

      Pages 95-95

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      Protocol

      Pages 97-107

      Development of a Single Locus Sequence Typing (SLST) Scheme for Typing Bacterial Species Directly from Complex Communities

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      Protocol

      Pages 109-137

      Reconstructing the Ancestral Relationships Between Bacterial Pathogen Genomes

  5. Bacterial Imaging Approaches and Related Techniques

    1. Front Matter

      Pages 139-139

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      Protocol

      Pages 141-159

      Making Fluorescent Streptococci and Enterococci for Live Imaging

    3. No Access

      Protocol

      Pages 161-172

      Computer Vision-Based Image Analysis of Bacteria

    4. No Access

      Protocol

      Pages 173-195

      Assessing Vacuolar Escape of Listeria Monocytogenes

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      Protocol

      Pages 197-209

      Immobilization Techniques of Bacteria for Live Super-resolution Imaging Using Structured Illumination Microscopy

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      Protocol

      Pages 211-217

      Negative Staining and Transmission Electron Microscopy of Bacterial Surface Structures

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      Protocol

      Pages 219-228

      Detection of Intracellular Proteins by High-Resolution Immunofluorescence Microscopy in Streptococcus pyogenes

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      Protocol

      Pages 229-241

      Antibody Guided Molecular Imaging of Infective Endocarditis

  6. Models for Studying Bacterial Pathogenesis

    1. Front Matter

      Pages 243-243

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      Protocol

      Pages 245-266

      The Zebrafish as a Model for Human Bacterial Infections

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      Protocol

      Pages 267-273

      Determining Platelet Activation and Aggregation in Response to Bacteria

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      Protocol

      Pages 275-284

      Killing Bacteria with Cytotoxic Effector Proteins of Human Killer Immune Cells: Granzymes, Granulysin, and Perforin

    5. No Access

      Protocol

      Pages 285-299

      In Vitro and In Vivo Biofilm Formation by Pathogenic Streptococci

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      Protocol

      Pages 301-315

      Murine Mycobacterium marinum Infection as a Model for Tuberculosis

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