Book Volume 1534 2017

Oncogene-Induced Senescence

Methods and Protocols

Editors:

ISBN: 978-1-4939-6668-4 (Print) 978-1-4939-6670-7 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Protocol

    Pages 1-15

    The Immortal Senescence

  3. No Access

    Protocol

    Pages 17-30

    Senescence Phenotypes Induced by Ras in Primary Cells

  4. No Access

    Protocol

    Pages 31-39

    Cellular Model of p21-Induced Senescence

  5. No Access

    Protocol

    Pages 41-52

    Detecting Markers of Therapy-Induced Senescence in Cancer Cells

  6. No Access

    Protocol

    Pages 53-68

    Genome-Wide miRNA Screening for Genes Bypassing Oncogene-Induced Senescence

  7. No Access

    Protocol

    Pages 69-78

    Detection of Dysfunctional Telomeres in Oncogene-Induced Senescence

  8. No Access

    Protocol

    Pages 79-87

    RT-qPCR Detection of Senescence-Associated Circular RNAs

  9. No Access

    Protocol

    Pages 89-98

    Autophagy Detection During Oncogene-Induced Senescence Using Fluorescence Microscopy

  10. No Access

    Protocol

    Pages 99-109

    Detecting the Senescence-Associated Secretory Phenotype (SASP) by High Content Microscopy Analysis

  11. No Access

    Protocol

    Pages 111-119

    Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells

  12. No Access

    Protocol

    Pages 121-125

    Using [U-13C6]-Glucose Tracer to Study Metabolic Changes in Oncogene-Induced Senescence Fibroblasts

  13. No Access

    Protocol

    Pages 127-137

    Detection of the Ubiquitinome in Cells Undergoing Oncogene-Induced Senescence

  14. No Access

    Protocol

    Pages 139-145

    Detection of Reactive Oxygen Species in Cells Undergoing Oncogene-Induced Senescence

  15. No Access

    Protocol

    Pages 147-153

    Detection of Senescent Cells by Extracellular Markers Using a Flow Cytometry-Based Approach

  16. No Access

    Protocol

    Pages 155-163

    Metabolic Changes Investigated by Proton NMR Spectroscopy in Cells Undergoing Oncogene-Induced Senescence

  17. No Access

    Protocol

    Pages 165-173

    Detection of Nucleotide Disbalance in Cells Undergoing Oncogene-Induced Senescence

  18. No Access

    Protocol

    Pages 175-184

    Senescence-Like Phenotypes in Human Nevi

  19. No Access

    Protocol

    Pages 185-198

    Detection of Oncogene-Induced Senescence In Vivo

  20. No Access

    Protocol

    Pages 199-210

    Detection of Senescence Markers During Mammalian Embryonic Development

  21. No Access

    Protocol

    Pages 211-218

    Induction and Detection of Oncogene-Induced Cellular Senescence in Drosophila

  22. Back Matter

    Pages 219-220