Book Volume 1527 2017

Hypertension

Methods and Protocols

Editors:

ISBN: 978-1-4939-6623-3 (Print) 978-1-4939-6625-7 (Online)

Table of contents (32 protocols)

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  1. Front Matter

    Pages i-xv

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    Protocol

    Pages 1-26

    Large-Scale Transcriptome Analysis

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    Protocol

    Pages 27-40

    Methods to Assess Genetic Risk Prediction

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    Protocol

    Pages 41-52

    Microarray Analysis of Hypertension

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    Protocol

    Pages 53-60

    Tissue Proteomics in Vascular Disease

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    Protocol

    Pages 61-68

    Urine Metabolomics in Hypertension Research

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    Protocol

    Pages 69-79

    Systems Biology Approach in Hypertension Research

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    Protocol

    Pages 81-99

    Measurement of Angiotensin Peptides: HPLC-RIA

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    Protocol

    Pages 101-115

    Measurement of Angiotensin Converting Enzyme 2 Activity in Biological Fluid (ACE2)

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    Protocol

    Pages 117-126

    Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate

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    Protocol

    Pages 127-137

    Measurement of Cardiac Angiotensin II by Immunoassays, HPLC-Chip/Mass Spectrometry, and Functional Assays

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    Protocol

    Pages 139-150

    Analysis of the Aldosterone Synthase (CYP11B2) and 11β-Hydroxylase (CYP11B1) Genes

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    Protocol

    Pages 151-161

    Dopaminergic Immunofluorescence Studies in Kidney Tissue

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    Protocol

    Pages 163-176

    Techniques for the Evaluation of the Genetic Expression, Intracellular Storage, and Secretion of Polypeptide Hormones with Special Reference to the Natriuretic Peptides (NPs)

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    Protocol

    Pages 177-187

    Intracellular Free Calcium Measurement Using Confocal Imaging

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    Protocol

    Pages 189-200

    Measuring T-Type Calcium Channel Currents in Isolated Vascular Smooth Muscle Cells

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    Protocol

    Pages 201-211

    In Vitro Analysis of Hypertensive Signal Transduction: Kinase Activation, Kinase Manipulation, and Physiologic Outputs

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    Protocol

    Pages 213-218

    In Vitro and In Vivo Approaches to Assess Rho Kinase Activity

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    Protocol

    Pages 219-232

    NADPH Oxidases and Measurement of Reactive Oxygen Species

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    Protocol

    Pages 233-249

    Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation

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    Protocol

    Pages 251-269

    Assessment of Caveolae/Lipid Rafts in Isolated Cells

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