Book Volume 1524 2017

Cell Cycle Synchronization

Methods and Protocols

Editors:

ISBN: 978-1-4939-6602-8 (Print) 978-1-4939-6603-5 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xv

  2. Cell Cycle Synchronization

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-27

      Overview of Cell Synchronization

  3. Physical Fractionation

    1. Front Matter

      Pages 29-29

    2. No Access

      Protocol

      Pages 31-52

      Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation

    3. No Access

      Protocol

      Pages 53-64

      Image Cytofluorometry for the Quantification of Ploidy and Endoplasmic Reticulum Stress in Cancer Cells

    4. No Access

      Protocol

      Pages 65-74

      Large-Scale Mitotic Cell Synchronization

  4. Chemical Blockade

    1. Front Matter

      Pages 75-75

    2. No Access

      Protocol

      Pages 77-95

      Synchronization and Desynchronization of Cells by Interventions on the Spindle Assembly Checkpoint

    3. No Access

      Protocol

      Pages 97-105

      Synchronization of Mammalian Cell Cultures by Serum Deprivation

    4. No Access

      Protocol

      Pages 107-119

      DNA Damage Response Resulting from Replication Stress Induced by Synchronization of Cells by Inhibitors of DNA Replication: Analysis by Flow Cytometry

    5. No Access

      Protocol

      Pages 121-147

      Chromosome Formation During Fertilization in Eggs of the Teleost Oryzias latipes

    6. No Access

      Protocol

      Pages 149-159

      Flow Cytometry Analysis of Cell Cycle and Specific Cell Synchronization with Butyrate

    7. No Access

      Protocol

      Pages 161-176

      Chemically Induced Cell Cycle Arrest in Perfusion Cell Culture

    8. No Access

      Protocol

      Pages 177-188

      Analysis of Nuclear Uracil DNA–Glycosylase (nUDG) Turnover During the Cell Cycle

    9. No Access

      Protocol

      Pages 189-201

      Synchronization of HeLa Cells

  5. Synchronization of Unicellular Organisms

    1. Front Matter

      Pages 203-203

    2. No Access

      Protocol

      Pages 205-213

      Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth

    3. No Access

      Protocol

      Pages 215-242

      Synchronization of Yeast

    4. No Access

      Protocol

      Pages 243-252

      Synchronization of Pathogenic Protozoans

  6. Synchronizing Mammalian and Transfected Cells

    1. Front Matter

      Pages 253-253

    2. No Access

      Protocol

      Pages 255-264

      Synchronization of In Vitro Maturation in Porcine Oocytes

  7. Synchronization of Plant Cells

    1. Front Matter

      Pages 265-265

    2. No Access

      Protocol

      Pages 267-285

      Detection of Changes in the Medicago sativa Retinoblastoma-Related Protein (MsRBR1) Phosphorylation During Cell Cycle Progression in Synchronized Cell Suspension Culture

  8. Synchronization of Embryonic Cells

    1. Front Matter

      Pages 287-287

    2. No Access

      Protocol

      Pages 289-298

      Nuclear Treatment and Cell Cycle Synchronization for the Purpose of Mammalian and Primate Somatic Cell Nuclear Transfer (SCNT)

  9. Hematopoietic Stem Cells

    1. Front Matter

      Pages 299-299

    2. No Access

      Protocol

      Pages 301-311

      Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation

  10. Clinical Study

    1. Front Matter

      Pages 313-313

    2. No Access

      Protocol

      Pages 315-327

      Intracellular Flow Cytometry Improvements in Clinical Studies

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