Book Volume 1515 2017

Cohesin and Condensin

Methods and Protocols

Editors:

ISBN: 978-1-4939-6543-4 (Print) 978-1-4939-6545-8 (Online)

Table of contents (16 protocols)

  1. Front Matter

    Pages i-xii

  2. In Vitro and In Vivo Systems to Dissect Molecular Mechanisms of Cohesin Regulation in Mitosis and Meiosis

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-21

      A Sister Chromatid Cohesion Assay Using Xenopus Egg Extracts

    3. No Access

      Protocol

      Pages 23-35

      An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complex

    4. No Access

      Protocol

      Pages 37-53

      Biochemical and Functional Assays of Human Cohesin-Releasing Factor Wapl

    5. No Access

      Protocol

      Pages 55-64

      Detection of Cohesin SUMOylation In Vivo

    6. No Access

      Protocol

      Pages 65-95

      Analysis of Meiotic Sister Chromatid Cohesion in Caenorhabditis elegans

    7. No Access

      Protocol

      Pages 97-111

      Protein and Chromosome Analysis in Mammalian Meiocytes

  3. The Roles of Cohesin in Gene Regulation, Chromatin Interactions, Ribosome Biogenesis, and DNA Repair

    1. Front Matter

      Pages 113-113

    2. No Access

      Protocol

      Pages 115-123

      Resolving the Genomic Localization of the Kollerin Cohesin-Loader Complex

    3. No Access

      Protocol

      Pages 125-139

      Measuring Sister Chromatid Cohesion Protein Genome Occupancy in Drosophila melanogaster by ChIP-seq

    4. No Access

      Protocol

      Pages 141-149

      A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis

    5. No Access

      Protocol

      Pages 151-176

      Methods to Study the Atypical Roles of DNA Repair and SMC Proteins in Gene Silencing

    6. No Access

      Protocol

      Pages 177-196

      Zebrafish as a Model to Study Cohesin and Cohesinopathies

    7. No Access

      Protocol

      Pages 197-216

      Analysis of Cohesin Function in Gene Regulation and Chromatin Organization in Interphase

    8. No Access

      Protocol

      Pages 217-226

      Using Fluorescent Reporters in Conjunction with Cytometry and Statistics to Assess Nuclear Accumulation of Ribosomal Proteins

    9. No Access

      Protocol

      Pages 227-242

      The Use of Laser Microirradiation to Investigate the Roles of Cohesins in DNA Repair

  4. Dissection of Condensin Function in Mitotic Chromosome Condensation and Gene Regulation

    1. Front Matter

      Pages 243-243

    2. No Access

      Protocol

      Pages 245-255

      A Protocol for Measuring Mitotic Chromosome Condensation Quantitatively in Fission Yeast Cells

    3. No Access

      Protocol

      Pages 257-271

      ChIP-seq Analysis of Condensin Complex in Cultured Mammalian Cells

  5. Back Matter

    Pages 273-276