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MicroRNA Profiling

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Sweta Rani

    1. Department of Science, Waterford Institute of Technology, Waterford, Ireland

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1509)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. MiRNA Biogenesis and Regulation of Diseases: An Overview

    • Anchal Vishnoi, Sweta Rani
    Pages 1-10

  3. Assessment of Basic Biological Functions Exerted by miRNAs

    • John Nolan, Raymond L. Stallings, Olga Piskareva
    Pages 11-16

  4. Extraction of miRNAs from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues

    • Karen Howe
    Pages 17-24

  5. MiRNA Isolation from Plants Rich in Polysaccharides and Polyphenols

    • K. K. Sabu, Fasiludeen Nadiya, Narayanannair Anjali
    Pages 25-36

  6. MicroRNA Profiling of Exosomes

    • Melissa Daly, Lorraine O’Driscoll
    Pages 37-46

  7. MiRNA Profiling in Human Induced Pluripotent Stem Cells

    • Erica Hennessy
    Pages 47-56

  8. MiRNA Expression in Cystic Fibrosis Bronchial Epithelial Cells

    • Irene K. Oglesby, Paul J. McKiernan
    Pages 57-69

  9. TaqMan Low Density Array: MicroRNA Profiling for Biomarker and Oncosuppressor Discovery

    • Keith O’Brien
    Pages 71-84

  10. Detection of MicroRNAs in Brain Slices Using In Situ Hybridization

    • Sean Quinlan, Christine Henke, Gary P. Brennan, David C. Henshall, Eva M. Jimenez-Mateos
    Pages 85-91

  11. Exosomal MicroRNA Discovery in Age-Related Macular Degeneration

    • Hanan Elshelmani, Sweta Rani
    Pages 93-113

  12. Profiling the MicroRNA Payload of Exosomes Derived from Ex Vivo Primary Colorectal Fibroblasts

    • Rahul Bhome, Rebecca Goh, Karen Pickard, Massimiliano Mellone, A. Emre Sayan, Alex Mirnezami
    Pages 115-122

  13. Circulating MicroRNAs in Cancer

    • Killian P. O’Brien, Eimear Ramphul, Linda Howard, William M. Gallagher, Carmel Malone, Michael J. Kerin et al.
    Pages 123-139

  14. Profiling Circulating miRNAs from the Plasma of Individuals with Metabolic Syndrome

    • Sadhbh O’Neill, Lorraine O’Driscoll
    Pages 141-149

  15. Manipulating MiRNA Expression to Uncover Hidden Functions

    • Sinéad T. Aherne, Nga T. Lao
    Pages 151-160

  16. Analysis of the Distribution Profiles of Circulating MicroRNAs by Asymmetrical Flow Field Flow Fractionation

    • Kenneth Flack, Luis A. Jimenez, Wenwan Zhong
    Pages 161-168

  17. MicroRNA Expression Profiling Using Agilent One-Color Microarray

    • Carmela Dell’Aversana, Cristina Giorgio, Lucia Altucci
    Pages 169-183

  18. A Multiplex Ligation Assay for miRNA Copy Number Profiling

    • Duncan Kilburn, Yunke Song, Tza-Huei Wang, Kelvin J. Liu
    Pages 185-193

  19. Practical Bioinformatics Analysis of MiRNA Data Using Online Tools

    • James A. L. Brown, Emer Bourke
    Pages 195-208

  20. Visualization and Analysis of MiRNA–Targets Interactions Networks

    • Luis E. León, Sebastián D. Calligaris
    Pages 209-220
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Keywords

About this book

This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocols discuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived product including formalin fixed paraffin embedded tissue and plant tissue. The last few chapters discuss isolation and characterization of exosomes from medium conditioned by cell lines, serum, and plasma specimens. This book also includes discussions of several software tools, such as miRandola, PicTar, DIANA, and miRWalk. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and cutting-edge, MicroRNA Profiling: Methods and Protocols is a valuable resource for anyone interested in the field of Micro RNAs.

Editors and Affiliations

  • Department of Science, Waterford Institute of Technology, Waterford, Ireland

    Sweta Rani

Bibliographic Information

  • Book Title: MicroRNA Profiling

  • Book Subtitle: Methods and Protocols

  • Editors: Sweta Rani

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6524-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6522-9Published: 09 November 2016

  • Softcover ISBN: 978-1-4939-8225-7Published: 20 June 2018

  • eBook ISBN: 978-1-4939-6524-3Published: 08 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 246

  • Number of Illustrations: 9 b/w illustrations, 32 illustrations in colour

  • Topics: Human Genetics

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