Book Volume 1412 2016

Endocannabinoid Signaling

Methods and Protocols

Editors:

ISBN: 978-1-4939-3537-6 (Print) 978-1-4939-3539-0 (Online)

Table of contents (28 protocols)

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  1. Front Matter

    Pages i-xi

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    Protocol

    Pages 1-8

    Need for Methods to Investigate Endocannabinoid Signaling

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    Protocol

    Pages 9-18

    Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues

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    Protocol

    Pages 19-26

    Determination of 2-Arachidonoylglycerol by μSPE-LC-MS/MS

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    Protocol

    Pages 27-40

    Analysis of Omega-3 Fatty Acid Derived N-Acylethanolamines in Biological Matrices

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    Protocol

    Pages 41-55

    Assay of CB1 Receptor Binding

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    Protocol

    Pages 57-63

    The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells

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    Protocol

    Pages 65-76

    Assay of TRPV1 Receptor Signaling

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    Protocol

    Pages 77-83

    A Functional Assay for GPR55: Envision Protocol

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    Protocol

    Pages 85-93

    The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells

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    Protocol

    Pages 95-101

    Assay of GTPγS Binding in Autoradiography

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    Protocol

    Pages 103-111

    Protocol to Study β-Arrestin Recruitment by CB1 and CB2 Cannabinoid Receptors

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    Protocol

    Pages 113-122

    Assay of NAT Activity

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    Protocol

    Pages 123-130

    Assay of NAPE-PLD Activity

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    Protocol

    Pages 131-136

    Assay of FAAH Activity

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    Protocol

    Pages 137-148

    Assay of NAAA Activity

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    Protocol

    Pages 149-156

    Assay of DAGLα/β Activity

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    Protocol

    Pages 157-168

    Assay of Monoacylglycerol Lipase Activity

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    Protocol

    Pages 169-178

    A Sensitive and Versatile Fluorescent Activity Assay for ABHD6

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    Protocol

    Pages 179-189

    A Sensitive and Versatile Fluorescent Activity Assay for ABHD12

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    Protocol

    Pages 191-203

    Assay of Endocannabinoid Uptake

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