Skip to main content
  • Book
  • © 2016

Proteomics in Systems Biology

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1394)

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
  2. Two Birds with One Stone: Parallel Quantification of Proteome and Phosphoproteome Using iTRAQ

    • Fiorella A. Solari, Laxmikanth Kollipara, Albert Sickmann, René P. Zahedi
    Pages 25-41
  3. A Targeted MRM Approach for Tempo-Spatial Proteomics Analyses

    • Annie Moradian, Tanya R. Porras-Yakushi, Michael J. Sweredoski, Sonja Hess
    Pages 75-85
  4. Targeted Phosphoproteome Analysis Using Selected/Multiple Reaction Monitoring (SRM/MRM)

    • Jun Adachi, Ryohei Narumi, Takeshi Tomonaga
    Pages 87-100
  5. Testing Suitability of Cell Cultures for SILAC-Experiments Using SWATH-Mass Spectrometry

    • Yvonne Reinders, Daniel Völler, Anja-K. Bosserhoff, Peter J. Oefner, Jörg Reinders
    Pages 101-108
  6. Tissue MALDI Mass Spectrometry Imaging (MALDI MSI) of Peptides

    • Birte Beine, Hanna C. Diehl, Helmut E. Meyer, Corinna Henkel
    Pages 129-150
  7. Ethyl Esterification for MALDI-MS Analysis of Protein Glycosylation

    • Karli R. Reiding, Emanuela Lonardi, Agnes L. Hipgrave Ederveen, Manfred Wuhrer
    Pages 151-162
  8. A Systems Approach to Understand Antigen Presentation and the Immune Response

    • Nadine L. Dudek, Nathan P. Croft, Ralf B. Schittenhelm, Sri H. Ramarathinam, Anthony W. Purcell
    Pages 189-209
  9. Profiling of Small Molecules by Chemical Proteomics

    • Kilian V. M. Huber, Giulio Superti-Furga
    Pages 211-218
  10. A Proteomic Workflow Using High-Throughput De Novo Sequencing Towards Complementation of Genome Information for Improved Comparative Crop Science

    • Reinhard Turetschek, David Lyon, Getinet Desalegn, Hans-Peter Kaul, Stefanie Wienkoop
    Pages 233-243
  11. From Phosphoproteome to Modeling of Plant Signaling Pathways

    • Maksim Zakhartsev, Heidi Pertl-Obermeyer, Waltraud X. Schulze
    Pages 245-259
  12. Interpretation of Quantitative Shotgun Proteomic Data

    • Elise Aasebø, Frode S. Berven, Frode Selheim, Harald Barsnes, Marc Vaudel
    Pages 261-273

About this book

This volume presents an overview of contemporary quantitative proteomics methods along with instructions on data interpretation, while providing examples on how to implement proteomics into systems biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Proteomics in Systems Biology: Methods and Protocols is a valuable resource for researchers who are interested in using proteomics techniques to help answer biological and medical questions.

Editors and Affiliations

  • University of Regensburg Institute of Functional Genomics, Regensburg, Germany

    Jörg Reinders

About the editor

Dr. Joerg Reinders Institute of Functional Genomics University Regensburg Joseph-Engert-Strasse 9 93053 Regensburg Germany Phone: +49 941 943 5004 Fax: +49 941 943 5019 E-Mail: joerg.reinders@klinik.uni-regensburg.de

Bibliographic Information

  • Book Title: Proteomics in Systems Biology

  • Book Subtitle: Methods and Protocols

  • Editors: Jörg Reinders

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3341-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3339-6Published: 23 December 2015

  • Softcover ISBN: 978-1-4939-8025-3Published: 27 March 2019

  • eBook ISBN: 978-1-4939-3341-9Published: 23 December 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 313

  • Number of Illustrations: 15 b/w illustrations, 61 illustrations in colour

  • Topics: Protein Science, Proteomics

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access