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Cytoskeleton Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Ray H. Gavin

    1. Brooklyn College, Brooklyn, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1365)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Imaging the Cytoskeleton in Live and Fixed Cells

    1. Front Matter

      Pages 1-1
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    2. Long-Term Live Cell Imaging of Cell Migration: Effects of Pathogenic Fungi on Human Epithelial Cell Migration

      • Torsten Wöllert, George M. Langford
      Pages 3-23

    3. Live-Cell Imaging of Mitochondria and the Actin Cytoskeleton in Budding Yeast

      • Ryo Higuchi-Sanabria, Theresa C. Swayne, Istvan R. Boldogh, Liza A. Pon
      Pages 25-62

    4. Imaging of the Actin Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells

      • Ryo Higuchi-Sanabria, Theresa C. Swayne, Istvan R. Boldogh, Liza A. Pon
      Pages 63-81

    5. Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells

      • Derek A. Applewhite, Christine A. Davis, Eric R. Griffis, Omar A. Quintero
      Pages 83-97

    6. Imaging Cytoskeleton Components by Electron Microscopy

      • Tatyana Svitkina
      Pages 99-118

    7. Purification and Localization of Intraflagellar Transport Particles and Polypeptides

      • Roger D. Sloboda
      Pages 119-137

    8. Fluorescence Imaging of the Cytoskeleton in Plant Roots

      • Julia Dyachok, Ana Paez-Garcia, Cheol-Min Yoo, Karuppaiah Palanichelvam, Elison B. Blancaflor
      Pages 139-153

    9. Microtubules in Plant Cells: Strategies and Methods for Immunofluorescence, Transmission Electron Microscopy, and Live Cell Imaging

      • Katherine Celler, Miki Fujita, Eiko Kawamura, Chris Ambrose, Klaus Herburger, Andreas Holzinger et al.
      Pages 155-184

  3. Cytoskeleton Dynamics

    1. Front Matter

      Pages 185-185
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    2. Basic Methods to Visualize Actin Filaments In Vitro Using Fluorescence Microscopy for Observation of Filament Severing and Bundling

      • Shoichiro Ono
      Pages 187-193

    3. An In Vitro Model System to Test Mechano-microbiological Interactions Between Bacteria and Host Cells

      • Luís Carlos Santos, Emilia Laura Munteanu, Nicolas Biais
      Pages 195-212

    4. Reconstitution of a Minimal Actin Cortex by Coupling Actin Filaments to Reconstituted Membranes

      • Sven K. Vogel
      Pages 213-223

    5. Use of Nanobodies to Localize Endogenous Cytoskeletal Proteins and to Determine Their Contribution to Cancer Cell Invasion by Using an ECM Degradation Assay

      • Isabel Van Audenhove, Jan Gettemans
      Pages 225-241

    6. Actin-Dynamics in Plant Cells: The Function of Actin-Perturbing Substances: Jasplakinolide, Chondramides, Phalloidin, Cytochalasins, and Latrunculins

      • Andreas Holzinger, Kathrin Blaas
      Pages 243-261

  4. Cell and Organelle Motility

    1. Front Matter

      Pages 263-263
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    2. Quantitative Motion Analysis in Two and Three Dimensions

      • Deborah J. Wessels, Daniel F. Lusche, Spencer Kuhl, Amanda Scherer, Edward Voss, David R. Soll
      Pages 265-292

    3. Measurement of Cell Motility Using Microgrooved Substrates

      • James H.-C. Wang, Guangyi Zhao, Bin Li
      Pages 293-299

    4. The Study of Cell Motility by Cell Traction Force Microscopy (CTFM)

      • James H.-C. Wang, Guangyi Zhao, Bin Li
      Pages 301-313
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Keywords

About this book

The third edition of this volume focuses on experimental models that are useful for investigating various aspects of cytoskeleton structure and function. Animal, plant, protist, and fungal models highlight twenty-four chapters that provide detailed protocols for live and fixed-cell imaging, dynamics of cytoskeleton components, cell and organelle motility, and genetics and proteomics. Written in the highly successful Methods in Molecular Biology series format, protocols in each chapter are up-to-date menus organized in a useful step-by-step format appropriate for novice and established investigators. Each chapter is equipped with a valuable notes section that provides a troubleshooting guide and helpful, and often unpublished, technical information aimed at ensuring success with implementation of the protocols.

Authoritative and thorough, Cytoskeleton Methods and Protocols, Third Edition helps researchers expand their understanding of cytoskeleton structure and function.

Editors and Affiliations

  • Brooklyn College, Brooklyn, USA

    Ray H. Gavin

Bibliographic Information

  • Book Title: Cytoskeleton Methods and Protocols

  • Editors: Ray H. Gavin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3124-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3123-1Published: 24 October 2015

  • Softcover ISBN: 978-1-4939-4975-5Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3124-8Published: 23 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIII, 431

  • Number of Illustrations: 53 b/w illustrations, 38 illustrations in colour

  • Topics: Cell Biology

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