Book Volume 1248 2015

Peptide Libraries

Methods and Protocols

Editors:

ISBN: 978-1-4939-2019-8 (Print) 978-1-4939-2020-4 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xvii

  2. Chemical Strategies for Library Synthesis

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-22

      Design, Synthesis, and Application of OB2C Combinatorial Peptide and Peptidomimetic Libraries

    3. No Access

      Protocol

      Pages 23-38

      Synthesis of Macrocyclic Organo-peptide Hybrids from Ribosomal Polypeptide Precursors via CuAAC-/Hydrazide-Mediated Cyclization

    4. No Access

      Protocol

      Pages 39-53

      Synthesis and Screening of One-Bead-One-Compound Cyclic Peptide Libraries

    5. No Access

      Protocol

      Pages 55-65

      Creating Site-Specific Isopeptide Linkages Between Proteins with the Traceless Staudinger Ligation

    6. No Access

      Protocol

      Pages 67-80

      Macrocyclic Templates for Library Synthesis of Peptido-Conjugates

    7. No Access

      Protocol

      Pages 81-91

      Synthesis and Alkylation of Aza-Gly-Pro Building Blocks of Peptidomimetic Libraries for Developing Prostaglandin F2α Receptor Modulators as Therapeutics to Inhibit Preterm Labor

  3. Chemistry and Genetically-Encoded Peptide Libraries

    1. Front Matter

      Pages 93-93

    2. No Access

      Protocol

      Pages 95-104

      A Fragment-Based Selection Approach for the Discovery of Peptide Macrocycles Targeting Protein Kinases

    3. No Access

      Protocol

      Pages 105-117

      Peptide and Peptide Library Cyclization via Bromomethylbenzene Derivatives

    4. No Access

      Protocol

      Pages 119-137

      Phage Selection of Bicyclic Peptides Based on Two Disulfide Bridges

    5. No Access

      Protocol

      Pages 139-153

      De Novo Discovery of Bioactive Cyclic Peptides Using Bacterial Display and Flow Cytometry

    6. No Access

      Protocol

      Pages 155-172

      Chemical Posttranslational Modification of Phage-Displayed Peptides

    7. No Access

      Protocol

      Pages 173-188

      Mapping Protein–Protein Interactions with Phage-Displayed Combinatorial Peptide Libraries and Alanine Scanning

    8. No Access

      Protocol

      Pages 189-199

      Identifying Reactive Peptides from Phage-Displayed Libraries

    9. No Access

      Protocol

      Pages 201-220

      The Best and the Brightest: Exploiting Tryptophan-Sensitized Tb3+ Luminescence to Engineer Lanthanide-Binding Tags

  4. Utility of Peptide Libraries

    1. Front Matter

      Pages 221-221

    2. No Access

      Protocol

      Pages 223-237

      Synthesis and Cell-Based Screening of One-Bead-One-Compound Peptide Libraries

    3. No Access

      Protocol

      Pages 239-247

      Screening Peptide Array Library for the Identification of Cancer Cell-Binding Peptides

    4. No Access

      Protocol

      Pages 249-266

      Next-Generation Sequencing of Phage-Displayed Peptide Libraries

    5. No Access

      Protocol

      Pages 267-276

      Maleimide-Based Method for Elaboration of Cysteine-Containing Peptide Phage Libraries

  5. Back Matter

    Pages 277-278