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  • Book
  • © 2014

Notch Signaling

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1187)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xi
  2. Introduction to Notch Signaling

    • Shinya Yamamoto, Karen L. Schulze, Hugo J. Bellen
    Pages 1-14
  3. Tracking Trafficking of Notch and Its Ligands in Mammalian Cells

    • Patricia Chastagner, Christel Brou
    Pages 87-100
  4. Visualizing Notch Signaling In Vivo in Drosophila Tissues

    • Benjamin E. Housden, Jinghua Li, Sarah J. Bray
    Pages 101-113
  5. Monitoring Notch Activity in the Mouse

    • Swananda Marathe, Lavinia Alberi
    Pages 115-129
  6. Notch Signaling Assays in Drosophila Cultured Cell Lines

    • Jinghua Li, Benjamin E. Housden, Sarah J. Bray
    Pages 131-141
  7. Visualization of Notch Signaling Oscillation in Cells and Tissues

    • Hiromi Shimojo, Yukiko Harima, Ryoichiro Kageyama
    Pages 169-179
  8. Proteomic Analysis of the Notch Interactome

    • K. G. Guruharsha, Kazuya Hori, Robert A. Obar, Spyros Artavanis-Tsakonas
    Pages 181-192
  9. Bacterial Expression and In Vitro Refolding of Limited Fragments of the Notch Receptor and Its Ligands

    • Pat Whiteman, Christina Redfield, Penny A. Handford
    Pages 193-208
  10. Assay to Probe Proteolytic Processing of Notch by γ-Secretase

    • Lutgarde Serneels, Ina Tesseur, Bart De Strooper
    Pages 223-229
  11. Identifying Direct Notch Transcriptional Targets Using the GSI-Washout Assay

    • Will Bailis, Yumi Yashiro-Ohtani, Warren S. Pear
    Pages 247-254

About this book

Notch Signaling: Methods and Protocols focuses on molecular, biochemical and bioinformatics aspects of Notch signaling. Chapters analyze the Notch interactome, post-translational modifications of Notch, ligand binding assays, methods to assess proteolytic cleavage and transcriptional targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Notch Signaling: Methods and Protocols is a valuable contribution to hundreds of labs and thousands of scientists who pursue this research area with vigor.

Editors and Affiliations

  • Dept.of Molecular and Human Genetics, Program in Developmental Biology, Dept. of Neuroscience, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, USA

    Hugo J. Bellen

  • Dept. of Molecular and Human Genetics, Program in Developmental Biology, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Baylor College of Medicine, Houston, USA

    Shinya Yamamoto

Bibliographic Information

  • Book Title: Notch Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Hugo J. Bellen, Shinya Yamamoto

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1139-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1138-7Published: 23 July 2014

  • Softcover ISBN: 978-1-4939-5510-7Published: 17 September 2016

  • eBook ISBN: 978-1-4939-1139-4Published: 22 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 351

  • Number of Illustrations: 13 b/w illustrations, 46 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access