Editors:
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1179)
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Table of contents (24 protocols)
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Front Matter
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Point Mutagenesis
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Front Matter
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Recombinatorial Methods
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Front Matter
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About this book
Directed Evolution Library Creation: Methods and Protocols, Second Edition presents user-friendly protocols for both proven strategies and cutting-edge approaches for the creation of mutant gene libraries for directed evolution. As well as experimental methods, information on current computational approaches is provided in a user-friendly format that will allow researchers to make informed choices without needing to comprehend the full technical details of each algorithm. Directed evolution has become a fundamental approach for engineering proteins to enhance activity and explore structure-function relationships, and has supported the rapid development of the field of synthetic biology over the last decade. Divided into three convenient sections, topics include point mutagenesis strategies, recombinatorial methods wherein genetic diversity is sourced from multiple parental genes that are combined via either homology-dependent or -independent techniques and a variety of computational methods to guide the design and analysis of mutant libraries. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Directed Evolution Library Creation: Methods and Protocols, Second Edition will serve as a reliable manual for both novice and experienced protein engineers and synthetic biologists and will enable further technical innovation and the exploitation of directed evolution for a deeper understanding of protein design and function.
Editors and Affiliations
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Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Brisbane, Australia
Elizabeth M.J. Gillam
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Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
Janine N. Copp
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School of Biological Sciences, Victoria University, Wellington, New Zealand
David Ackerley
Bibliographic Information
Book Title: Directed Evolution Library Creation
Book Subtitle: Methods and Protocols
Editors: Elizabeth M.J. Gillam, Janine N. Copp, David Ackerley
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1053-3
Publisher: Springer New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-1052-6Published: 24 July 2014
Softcover ISBN: 978-1-4939-5329-5Published: 17 September 2016
eBook ISBN: 978-1-4939-1053-3Published: 23 July 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XI, 369
Number of Illustrations: 44 b/w illustrations, 19 illustrations in colour
Topics: Human Genetics, Evolutionary Biology, Genetic Engineering