Fluorescence Spectroscopy in Biology

Advanced Methods and their Applications to Membranes, Proteins, DNA, and Cells

ISBN: 978-3-540-22338-2 (Print) 978-3-540-27004-1 (Online)

Table of contents (16 chapters)

  1. Fluorescence Spectroscopy: Basics and Advanced Approaches

    1. No Access

      Book Chapter

      Pages 3-29

      Basics of Fluorescence Spectroscopy in Biosciences

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      Book Chapter

      Pages 30-48

      Pulse and Phase Fluorometries: An Objective Comparison

    3. No Access

      Book Chapter

      Pages 49-55

      Non-Exponential Fluorescence of Electronically Coupled Donors Contains Distance Information

    4. No Access

      Book Chapter

      Pages 56-70

      Fluorescence Nanotomography: Recent Progress, Constraints and Opportunities

    5. No Access

      Book Chapter

      Pages 71-78

      Solvent Relaxation as a Tool for Probing Micro-Polarity and -Fluidity

    6. No Access

      Book Chapter

      Pages 79-103

      Total Internal Reflection Fluorescence Microscopy: Applications in Biophysics

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      Book Chapter

      Pages 104-130

      Single Molecule Spectroscopy: Basics and Applications

  2. Application of Fluorescence Spectroscopy to Biological Membranes

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      Book Chapter

      Pages 133-149

      Raft Microdomains in Model Membranes as Revealed by Fluorescence Quenching

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      Book Chapter

      Pages 150-159

      The Lateral Structure of Lipid Membranes as Seen by Fluorescence Microscopy

  3. Application of Fluorescence Spectroscopy to Protein Studies

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      Book Chapter

      Pages 163-179

      Protein Dynamics and Protein Folding Dynamics Revealed by Time-Resolved Fluorescence

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      Book Chapter

      Pages 180-197

      Time-Resolved Fluorescence and Two-Photon FCS Investigation of the Interaction of HIV-1 Nucleocapsid Protein with Hairpin Loop Oligonucleotides

  4. Application of Fluorescence Spectroscopy to DNA and Drug Delivery

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      Book Chapter

      Pages 201-228

      Fluorescence Techniques in Non-Viral Gene Therapy

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      Book Chapter

      Pages 229-242

      Fluorescence Applications in Targeted Drug Delivery

  5. Fluorescence Spectroscopy in cells: FCS and Quantum Dots

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      Book Chapter

      Pages 245-262

      Fluorescence Correlation Spectroscopy in Cell Biology

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      Book Chapter

      Pages 263-274

      Fluorescent Quantum Dots: Properties and Applications

    3. No Access

      Book Chapter

      Pages 275-287

      Heat Stress of Cancer Cells: Fluorescence Imaging of Structural Changes with Quantum Dots 605 and Alexa 488