1998

Molecular Tools for Screening Biodiversity

Plants and Animals

Editors:

ISBN: 978-94-010-6496-5 (Print) 978-94-009-0019-6 (Online)

Table of contents (83 chapters)

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  1. Front Matter

    Pages i-xxiv

  2. DNA Extraction

    1. Front Matter

      Pages 1-1

    2. DNA Extraction: widely applicable methods

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        Book Chapter

        Pages 5-9

        Isolation of Animal Cellular Total DNA

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        Book Chapter

        Pages 10-14

        Plant Total DNA Extraction

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        Book Chapter

        Pages 15-17

        Chloroplast DNA Extraction Procedure for Species High in Phenolics and Polysaccharides

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        Book Chapter

        Pages 18-21

        Quantification of DNA

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        Book Chapter

        Pages 22-24

        Miniprep Procedures for the Isolation of Plant DNA

      DNA Extraction: methods for difficult species/tissues

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        Book Chapter

        Pages 27-31

        Special DNA Extraction Methods for Some Animal Species

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        Pages 32-35

        Methods for Difficult Plant Species/Tissues

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        Book Chapter

        Pages 36-37

        DNA Maxipreparations: A Comparison of Protocols for Rapid Extraction of Good Quality DNA from Recalcitrant Species

      Preserved specimens

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        Book Chapter

        Pages 41-45

        Isolation of DNA from Preserved Specimens

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        Pages 46-48

        Field Collection: Animals

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        Pages 49-50

        Field Collection: Plants

      DNA Extraction using anion-exchange chromatography and silica-gel based membranes

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        Book Chapter

        Pages 53-54

        DNA Extraction Using Anion-Exchange Chromatography and Silica-Gel Based Membranes

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        Book Chapter

        Pages 54-59

        Isolation of Nucleic Acids Using Anion-Exchange Chromatography: QIAGEN-tip Based Methods

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        Book Chapter

        Pages 59-63

        Isolation of Nucleic Acids Using Silica-Gel Based Membranes: Methods Based on the Use of QIAamp Spin Columns

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        Book Chapter

        Pages 64-66

        Isolation of Total RNA from Animal Tissue Using Anion-Exchange Chromatography (QIAGEN-tips)

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        Book Chapter

        Pages 67-70

        Isolation of Total RNA Using Silica-Gel Based Membranes

  3. Basic Screening Methods

    1. Front Matter

      Pages 71-71

    2. Isozymes

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        Book Chapter

        Pages 75-81

        Isozymes

      RFLP analysis

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        Book Chapter

        Pages 85-95

        RFLP Analysis

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        Book Chapter

        Pages 96-97

        Nonradioactive Probes

      DNA fingerprinting with VNTR sequences

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        Book Chapter

        Pages 101-108

        DNA Fingerprinting with VNTR Sequences

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