Isotope labeling in Biomolecular NMR

Editors:

ISBN: 978-94-007-4953-5 (Print) 978-94-007-4954-2 (Online)

Table of contents (11 chapters)

  1. Front Matter

    Pages i-viii

  2. PROTEINS

    1. Front Matter

      Pages 1-1

    2. No Access

      Chapter

      Pages 3-15

      Isotope Labeling Methods for Large Systems

    3. No Access

      Chapter

      Pages 17-33

      Segmental Labeling to Study Multidomain Proteins

    4. No Access

      Chapter

      Pages 35-62

      Isotope Labeling for Solution and Solid-State NMR Spectroscopy of Membrane Proteins

    5. No Access

      Chapter

      Pages 63-82

      Isotope Labeling Methods for Relaxation Measurements

    6. No Access

      Chapter

      Pages 83-93

      Stereo-Array Isotope Labeling Method for Studying Protein Structure and Dynamics

    7. No Access

      Chapter

      Pages 95-118

      Amino Acid Selective Labeling and Unlabeling for Protein Resonance Assignments

  3. NUCLEIC ACIDS

    1. Front Matter

      Pages 119-119

    2. No Access

      Chapter

      Pages 121-144

      Isotope Labeling and Segmental Labeling of Larger RNAs for NMR Structural Studies

  4. METABOLOMICS

    1. Front Matter

      Pages 145-145

    2. No Access

      Chapter

      Pages 147-164

      Isotope Enhanced Approaches in Metabolomics

  5. EXPRESSION SYSTEMS

    1. Front Matter

      Pages 165-165

    2. No Access

      Chapter

      Pages 167-177

      Cell-Free Protein Synthesis Using E. coli Cell Extract for NMR Studies

    3. No Access

      Chapter

      Pages 179-196

      Isotope Labeling in Insect Cells

    4. No Access

      Chapter

      Pages 197-211

      Mammalian Expression of Isotopically Labeled Proteins for NMR Spectroscopy

  6. Back Matter

    Pages 213-219