Book Volume 5 2012

Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009


ISBN: 978-94-007-0883-9 (Print) 978-94-007-0884-6 (Online)

Table of contents (119 chapters)

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  1. Front Matter

    Pages i-xliv

  2. Synthetic and Molecular Biology

    1. Front Matter

      Pages 1-1

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      Pages 3-11

      MicroRNAs as Potential Engineering Targets for Improvement of CHO Cell Production Phenotypes

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      Pages 13-18

      Are Clones Really Unstable?

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      Pages 19-23

      An Optimised Transfection Platform for the Epi-CHO Transient Expression System in Serum-free Media

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      Pages 25-29

      Using the “OMICS” Technologies as Complementary Tools to Study the Molecular Mechanisms Involved with the Adaptation of Myeloma Cell Line to Protein-Free Medium

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      Pages 31-35

      Galectin-3 Over-Expression Enhances Survival and Recombinant Protein Expression in Mammalian Cells

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      Pages 37-41

      Metabolomic Analysis of CHO Cultures with Different Growth Characteristics – Development of a Metabolite Extraction Protocol for Suspension Adapted Mammalian Cells

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      Pages 43-46

      Cell Lines in Four Weeks with the CEMAX® System

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      Pages 47-51

      Analysis of Protein Expression via Alternate 3’ Untranslated Region (UTR) Signals Through the Use of Site Specific Recombination

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      Pages 53-56

      A Case Study in Clone Screening: A Comprehensive Approach for a Product With High Projected Market Demand

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      Pages 57-60

      An Improved Clone Selection Method

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      Pages 61-65

      Dissecting the Mechanism of Action of BHRF1 for the Protection Against Apoptosis in MAb-Producing Cell Lines

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      Pages 67-70

      Qualification of Bench and Pilot-Scale Bioreactors as Models of Commercial Systems

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      Pages 71-75

      Quantification of Polyethylenimine in Transient Gene Expression: On the Way to GMP Compliance

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      Pages 77-83

      Implications of Vector Fragmentation and Initial Production Levels in Production Cell Line Development Using MTX

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      Pages 85-90

      Characterizing the Expression Stability in Different Phenotypes of Recombinant NS0 Myeloma Cell Lines

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      Pages 91-94

      A Transient Gene Expression Process with Recombinant Antibody Titer of 0.5 g/L in CHO Cells

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      Pages 95-107

      Proteomic Profiling of Temperature-Shifted CHO Cells to Identify Genes that Impact on Both Cell Growth and Recombinant Protein Productivity in Suspension Culture

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      Pages 109-113

      Stabilization of Protein Expression in Mammalian Cells Employing a Toxin/Antitoxin Based Strategy

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      Pages 115-119

      Novel Cell Lines For Bioprocessing: Friend or Foe?

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      Pages 121-124

      Transgene mRNA Levels and Stability are Key Factors to Enhance Transient Gene Expression in CHO DG44 Cells

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