2011

Sample Preparation in Biological Mass Spectrometry

Editors:

ISBN: 978-94-007-0758-0 (Print) 978-94-007-0828-0 (Online)

Table of contents (49 chapters)

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  1. Front Matter

    Pages i-xxix

  2. Traditional And Improved Techniques In Sample Preparation For Proteomics

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Chapter

      Pages 3-17

      Gel-Based and Gel-Free Sample Preparation for LC-MS/MS Analysis

    3. No Access

      Book Chapter

      Pages 19-40

      Manipulating the Mass Spectrometric Properties of Peptides through Selective Chemical Modification

    4. No Access

      Book Chapter

      Pages 41-60

      Sample Preparation for 2D Electrophoresis and CE-Based Proteomics

    5. No Access

      Book Chapter

      Pages 61-75

      Filtration as a Sample Preparation Technique Prior to Mass Spectrometry: Selecting the Right Filtration Device

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      Book Chapter

      Pages 77-90

      Pressure-Assisted Lysis of Mammalian Cell Cultures Prior to Proteomic Analysis

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      Book Chapter

      Pages 91-105

      Multiplexed Preparation of Biological Samples for Mass Spectrometry Using Gel Electrophoresis

  3. Methods For Improved Proteolytic Digestion

    1. Front Matter

      Pages 107-107

    2. No Access

      Book Chapter

      Pages 109-124

      Development of an On-Bead Digestion Procedure for Immunoprecipitated Proteins

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      Book Chapter

      Pages 125-139

      Ultra-Fast Sample Preparation for High-Throughput Proteomics

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      Book Chapter

      Pages 141-155

      Exploring the Capabilities of the Protein Identification by Unconventional Sample Preparation Approaches: LC/MALDI/On-Target Digestion Approach and High Pressure-Assisted In-Gel Tryptic Digestion

  4. Methods For Tissue, Cell, And Organelle Preparation And Analysis

    1. Front Matter

      Pages 157-157

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      Book Chapter

      Pages 159-170

      Sample Preparation of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue for Proteomic Analyses

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      Book Chapter

      Pages 171-195

      Brain Proteomics: Sample Preparation Techniques for the Analysis of Rat Brain Samples Using Mass Spectrometry

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      Book Chapter

      Pages 197-214

      Subcellular Fractionation Using Step-Wise Density Extraction for Mass Spectrometry Analysis

  5. 2D Gel-Based Proteomics

    1. Front Matter

      Pages 215-215

    2. No Access

      Book Chapter

      Pages 217-242

      Two-Dimensional Polyacrylamide Gel Electrophoresis

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      Book Chapter

      Pages 243-253

      Quantitative Intact Proteomic Strategies to Detect Changes in Protein Modification and Genomic Variation

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      Book Chapter

      Pages 255-266

      Two-Dimensional Non-denaturing Gel Electrophoresis for Characterization of Proteins in Multi-molecular Particles by Mass Spectrometry

  6. Sample Preparation And Analysis Techniques For Biological Fluids And Biomarker Discovery

    1. Front Matter

      Pages 267-267

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      Book Chapter

      Pages 269-289

      Preanalytical Variables for Plasma and Serum Proteome Analyses

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      Book Chapter

      Pages 291-326

      Biomarker Discovery in Biological Fluids

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      Book Chapter

      Pages 327-360

      Sample Handling of Body Fluids for Proteomics

  7. Sample Preparation Methods In Plant Proteomics

    1. Front Matter

      Pages 361-361

    2. No Access

      Book Chapter

      Pages 363-380

      Quantitative Plant Proteomics Using Hydroponic Isotope Labeling of Entire Plants (HILEP)

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      Book Chapter

      Pages 381-409

      Efficient Strategies for Analysis of Low Abundance Proteins in Plant Proteomics

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