Volume 4 2010

Cells and Culture

Proceedings of the 20th ESACT Meeting, Dresden, Germany, June 17-20, 2007

Editors:

ISBN: 978-90-481-3418-2 (Print) 978-90-481-3419-9 (Online)

Table of contents (152 chapters)

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  1. Front Matter

    Pages i-liv

  2. Single-Cell Analysis and Engineering

    1. Front Matter

      Pages 1-1

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      Pages 3-8

      Targeted Gene Knockdown Effects on Recombinant Protein Secretion in CHO Cells

    3. Book Chapter

      Pages 9-12

      RNA Silencing Suppressors Boost the Production of Recombinant Proteins and Viruses

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      Pages 13-18

      Towards the Use of CHO Produced Recombinant Extracellular Matrix Proteins as Bioactive Elements in a 3-D Scaffold for Tissue Engineering

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      Pages 19-23

      Transient Gene Expression in Chinese Hamster Ovary Cells at Low Temperature – The Effects of Cold-Induced Proteins and an mRNA Regulatory Element

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      Pages 25-28

      Single-Cell Approach in Influenza Vaccine Production: Apoptosis and Virus Protein Production

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      Pages 29-34

      Chondrogenic Differentiation of Human Mesenchymal Stem Cells During Multiple Subcultivation

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      Pages 35-39

      Cell Xpress™-Assisted Analysis of Clone Stability in Recombinant Chinese Hamster Ovary Cells

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      Pages 41-44

      An Evaluation of the Intrinsic IgG Production Capabilities of Different Chinese Hamster Ovary Parental Cell Lines

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      Pages 45-48

      Cell Xpress™ Technology Facilitates High-Producing Chinese Hamster Ovary Cell Line Generation Using Glutamine Synthetase Gene Expression System

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      Pages 49-55

      CHO-DG44 Cell Line Development by FLP-Targeting – High Level Glycoprotein Expression with Significantly Decreased Time Lines

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      Pages 57-62

      Transgene Copy Number – Impact on Clone Performance

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      Pages 63-69

      Single Use Bioreactors: Expressing Protein in Mammalian Cell Suspension

  3. Applied Integrative Physiology

    1. Front Matter

      Pages 71-71

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      Pages 73-78

      Gene Modified Hematopoietic Stem Cells for the Treatment of Primary Immunodeficiency Diseases

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      Pages 79-88

      An Avian Cell Line for Production of Highly Attenuated Viral Vectors

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      Pages 89-93

      Microelectronic Cellular Vitality Monitoring

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      Pages 95-99

      IFN-γ Glycosylation Macroheterogeneity, Energetic Cell Status and Medium Composition During CHO Cell Cultures

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      Pages 101-106

      Modelling of Neural Metabolism Using 13C-NMR Spectroscopy and Metabolic Flux Analysis

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      Pages 107-111

      Influence of Glucose and Glutamine Concentration on Metabolism of rCHO Cells

  4. Speed and Intensification in Bioprocess Development

    1. Front Matter

      Pages 113-113

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      Pages 115-119

      Delivery of Biomolecules with Non-Viral Vectors

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      Pages 121-128

      Circumventing the “Pay Now or Pay Later” Dilemma: Strategies for Achieving Process Development with Speed and Long-Term Potential

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