Advanced Methods in Structural Biology

Editors:

ISBN: 978-4-431-56028-9 (Print) 978-4-431-56030-2 (Online)

Table of contents (17 protocols)

  1. Front Matter

    Pages i-x

  2. Protein Expression

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-23

      Expression in Bacteria and Refolding

    3. No Access

      Protocol

      Pages 25-45

      Expression of Proteins in Insect and Mammalian Cells

    4. No Access

      Protocol

      Pages 47-65

      Application of MultiBac System to Large Complexes

    5. No Access

      Protocol

      Pages 67-81

      Purification Using Affinity Tag Technology

    6. No Access

      Protocol

      Pages 83-102

      Cell-Free Protein Production for Structural Biology

  3. Purification and Crystallization of Membrane Proteins

    1. Front Matter

      Pages 103-103

    2. No Access

      Protocol

      Pages 105-122

      Overview of Membrane Protein Purification and Crystallization

    3. No Access

      Protocol

      Pages 123-135

      Cell-Free Synthesis of Membrane Proteins

  4. Crystallization and Crystal Engineering

    1. Front Matter

      Pages 137-137

    2. No Access

      Protocol

      Pages 139-151

      Screening of Cryoprotectants and the Multistep Soaking Method

    3. No Access

      Protocol

      Pages 153-161

      Protein Modification for Crystallization

  5. Interaction Analysis

    1. Front Matter

      Pages 163-163

    2. No Access

      Protocol

      Pages 165-183

      Analytical Ultracentrifugation

    3. No Access

      Protocol

      Pages 185-198

      Mass Spectrometry

    4. No Access

      Protocol

      Pages 199-226

      Frontal Gel Filtration

    5. No Access

      Protocol

      Pages 227-237

      Surface Plasmon Resonance

  6. Advanced Methods for Structural Analyses

    1. Front Matter

      Pages 239-239

    2. No Access

      Protocol

      Pages 241-273

      Structural Biology with Microfocus Beamlines

    3. No Access

      Protocol

      Pages 275-292

      Structural Biology and Electron Microscopy

    4. No Access

      Protocol

      Pages 293-314

      Structure Determination Software for Macromolecular X-Ray Crystallography

    5. No Access

      Protocol

      Pages 315-340

      NMR Structural Biology Using Paramagnetic Lanthanide Probe