2011

Guidelines for Molecular Analysis in Archive Tissues

Editors:

ISBN: 978-3-642-17889-4 (Print) 978-3-642-17890-0 (Online)

Table of contents (52 chapters)

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  1. Front Matter

    Pages i-xvi

  2. Archive Tissues

    1. Front Matter

      Pages 1-1

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      Book Chapter

      Pages 3-4

      Archive Tissues

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      Book Chapter

      Pages 5-11

      Preanalytical Time Interval (PATI) and Fixation

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      Book Chapter

      Pages 13-18

      Formalin-Free Fixatives

  3. Dissection of Tissue Components

    1. Front Matter

      Pages 19-19

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      Book Chapter

      Pages 21-22

      Manual Microdissection

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      Pages 23-26

      Tissue Microarray (TMA)

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      Pages 27-29

      Laser Capture Microdissection (LCM)

  4. Extraction, Purification, Quantification and Quality Assessment of Nucleic Acids

    1. Front Matter

      Pages 31-31

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      Book Chapter

      Pages 33-36

      DNA Extraction from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues

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      Book Chapter

      Pages 37-39

      DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues (FFPE) (from Small Fragments of Tissues or Microdissected Cells)

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      Pages 41-43

      Fast Protocol for DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues

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      Pages 45-54

      DNA Extraction from Blood and Forensic Samples

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      Pages 55-56

      Restoration and Reconstruction of DNA Length

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      Book Chapter

      Pages 57-61

      RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues

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      Book Chapter

      Pages 63-66

      RNA Extraction from Decalcified and Non-decalcified Formalin-Fixed Paraffin-Embedded Tissues

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      Book Chapter

      Pages 67-69

      RNA Temperature Demodification

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      Book Chapter

      Pages 71-74

      MicroRNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues

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      Book Chapter

      Pages 75-79

      Quantification of Nucleic Acids

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      Book Chapter

      Pages 81-85

      Integrity Assessment of Nucleic Acids

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      Book Chapter

      Pages 87-90

      DNase Treatment of RNA

  5. Reverse Transcription

    1. Front Matter

      Pages 91-91

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      Book Chapter

      Pages 93-97

      Reverse Transcription

  6. Nucleic Acid Amplification

    1. Front Matter

      Pages 99-99

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      Book Chapter

      Pages 101-103

      General Protocol for End-Point PCR

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