2010

MicroRNA Expression Detection Methods

Authors:

ISBN: 978-3-642-04927-9 (Print) 978-3-642-04928-6 (Online)

Table of contents (33 chapters)

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  1. Front Matter

    Pages i-xx

  2. General Remarks

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Chapter

      Pages 3-64

      Detection, Profiling, and Quantification of miRNA Expression

  3. miRNA Microarray Methods

    1. Front Matter

      Pages 66-66

    2. No Access

      Book Chapter

      Pages 67-79

      Microarray and Its Variants for miRNA Profiling

  4. Northern Blotting Methods

    1. Front Matter

      Pages 82-82

    2. No Access

      Book Chapter

      Pages 83-100

      Northern Blotting and Its Variants for Detecting Expression and Analyzing Tissue Distribution of miRNAs

  5. In Situ Hybridization Methods

    1. Front Matter

      Pages 102-102

    2. No Access

      Book Chapter

      Pages 103-128

      In Situ Hybridization and Its Variants for Detecting Expression and Analyzing Cellular Distribution of miRNAs

  6. Real-Time RT-PCR Methods

    1. Front Matter

      Pages 130-130

    2. No Access

      Book Chapter

      Pages 131-140

      End-Point Stem-Loop Real-Time RT-PCR for miRNA Quantification

    3. No Access

      Book Chapter

      Pages 141-146

      miR-Q RT-PCR for miRNA Quantification

    4. No Access

      Book Chapter

      Pages 147-151

      Poly(A)-Tailed Universal Reverse Transcription

    5. No Access

      Book Chapter

      Pages 153-157

      Multiplexing RT-PCR for High-Throughput miRNA Profiling

    6. No Access

      Book Chapter

      Pages 159-170

      miRNA Amplification Profiling (mRAP)

  7. Cloning Methods

    1. Front Matter

      Pages 172-172

    2. No Access

      Book Chapter

      Pages 173-188

      miRNA Serial Analysis of Gene Expression (miRAGE or SAGE)

  8. Nanoparticle Methods

    1. Front Matter

      Pages 190-190

    2. No Access

      Book Chapter

      Pages 191-198

      Electrocatalytic Nanoparticle Tags Technique for High-Sensitivity miRNA Expression Analysis

    3. No Access

      Book Chapter

      Pages 199-206

      Nanoparticle-Amplified SPR Imaging for High-Sensitivity miRNA Profiling

    4. No Access

      Book Chapter

      Pages 207-215

      Conducting Polymer Nanowires Technique for High-Sensitivity miRNA Expression Analysis

    5. No Access

      Book Chapter

      Pages 217-225

      Gold Nanoparticle Probe Method for miRNA Quantification

  9. Other Methods

    1. Front Matter

      Pages 228-228

    2. No Access

      Book Chapter

      Pages 229-239

      Splinted Ligation Method

    3. No Access

      Book Chapter

      Pages 241-247

      Padlock-Probes and Rolling-Circle Amplification

    4. No Access

      Book Chapter

      Pages 249-255

      Invader Assay

    5. No Access

      Book Chapter

      Pages 257-265

      Single Molecule Method

    6. No Access

      Book Chapter

      Pages 267-274

      Enzymatic Method

    7. No Access

      Book Chapter

      Pages 275-280

      Surface-Enhanced Raman Spectroscopy Method

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