Protein Engineering


ISBN: 978-3-540-70937-4 (Print) 978-3-540-70941-1 (Online)

Table of contents (12 chapters)

  1. Front Matter

    Pages i-xi

  2. Chapter

    Pages 1-27

    Understanding Enzyme Mechanism through Protein Chimeragenesis

  3. Chapter

    Pages 29-64

    Chemical Protein Engineering: Synthetic and Semisynthetic Peptides and Proteins

  4. Chapter

    Pages 65-96

    Native Chemical Ligation: SemiSynthesis of Post-translationally Modified Proteins and Biological Probes

  5. Chapter

    Pages 97-125

    Chemical Methods for Mimicking Post-Translational Modifications

  6. Chapter

    Pages 127-153

    Noncanonical Amino Acids in Protein Science and Engineering

  7. Chapter

    Pages 155-203

    Fidelity Mechanisms of the Aminoacyl-tRNA Synthetases

  8. Chapter

    Pages 205-229

    Specialized Components of the Translational Machinery for Unnatural Amino Acid Mutagenesis: tRNAs, Aminoacyl-tRNA Synthetases, and Ribosomes

  9. Chapter

    Pages 231-254

    In Vivo Studies of Receptors and Ion Channels with Unnatural Amino Acids

  10. Chapter

    Pages 255-270

    Synthesis of Modified Proteins Using Misacylated tRNAs

  11. Chapter

    Pages 271-290

    Cell-Free Synthesis of Proteins with Unnatural Amino Acids. The PURE System and Expansion of the Genetic Code

  12. Chapter

    Pages 291-313

    Engineering Nucleobases and Polymerases for an Expanded Genetic Alphabet

  13. Chapter

    Pages 315-337

    Understanding Membrane Proteins. How to Design Inhibitors of Transmembrane Protein—Protein Interactions

  14. Back Matter

    Pages 339-347