2009

Fluorescence In Situ Hybridization (FISH) — Application Guide

Editors:

ISBN: 978-3-540-70580-2 (Print) 978-3-540-70581-9 (Online)

Table of contents (36 protocols)

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  1. Front Matter

    Pages i-xviii

  2. Molecular Cytogenetics, Probes and Equipment

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      Pages 3-21

      Molecular Cytogenetic Applications in Diagnostics and Research: An Overview

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      Pages 23-34

      Molecular Cytogenetics: The Standard FISH and PRINS Procedure

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      Pages 35-52

      Generation of Paint Probes by Flow-Sorted and Microdissected Chromosomes

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      Pages 53-60

      ISH Probes Derived from BACs, Including Microwave Treatment for Better FISH Results

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      Pages 61-66

      PNA-FISH Technique for In Situ Assessment of Aneuploidy

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      Pages 67-73

      Oligonucleotide FISH Probes

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      Pages 75-84

      Microscopy and Imaging Systems

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      Pages 85-97

      Optical Filters for FISH

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      Pages 99-109

      FISH With and Without COT1 DNA

  3. FISH Applications in Different Tissue Preparations

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      Pages 113-122

      Pre- and Postnatal Diagnostics and Research on Peripheral Blood, Chorion, Amniocytes, and Fibroblasts

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      Pages 123-130

      Prenatal Diagnostics on Uncultured Amniocytes

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      Pages 131-136

      Tumorcytogenetic Diagnostics and Research on Uncultured Blood or Bone Marrow and Smears

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      Pages 137-140

      Application of FISH to Archival Cytogenetic Slides

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      Pages 141-146

      Characterization of Mosaicism in Various Easy-to-Acquire Body Tissues Such as Buccal Smears, Hair Root Cells or Urine

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      Pages 147-156

      Characterization of Archived Formalin-Fixed/Paraffin-Embedded or Cryofixed Tissue, Including Nucleus Extraction

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      Pages 157-172

      FISH on Sperms, Spermatocytes and Oocytes

  4. Characterization of Marker Chromosomes

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      Pages 175-181

      Multiplex FISH and Spectral Karyotyping

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      Pages 183-192

      Tips and Tricks for mFISH

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      Pages 193-216

      Simultaneous Fluorescence Immunostaining and FISH

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      Pages 217-230

      Color-Changing Karyotyping (CCK), an M-FISH/SKY Alternative

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