Volume 1130 2014

Skeletal Development and Repair

Methods and Protocols

Editors:

ISBN: 978-1-62703-988-8 (Print) 978-1-62703-989-5 (Online)

Table of contents (25 protocols)

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  1. Front Matter

    Pages i-x

  2. Understanding Mouse Skeletal Development and Repair

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-12

      Overview of Skeletal Development

    3. No Access

      Book Protocol

      Pages 13-31

      Overview of Skeletal Repair (Fracture Healing and Its Assessment)

  3. Skeletal Repair, Transplantations, and Organ Cultures

    1. Front Matter

      Pages 33-33

    2. No Access

      Book Protocol

      Pages 35-44

      Generation of Closed Transverse Fractures in Small Animals

    3. No Access

      Book Protocol

      Pages 45-59

      The Murine Femoral Bone Graft Model and a Semiautomated Histomorphometric Analysis Tool

    4. No Access

      Book Protocol

      Pages 61-72

      Surgical Induction of Posttraumatic Osteoarthritis in the Mouse

    5. No Access

      Book Protocol

      Pages 73-88

      A Mouse Model of Flexor Tendon Repair

    6. No Access

      Book Protocol

      Pages 89-97

      Cartilage Explant Cultures

    7. No Access

      Book Protocol

      Pages 99-110

      Renal Capsule Transplantations to Assay Skeletal Angiogenesis

  4. Histological and Molecular Analyses on Whole Tissues and Tissue Sections

    1. Front Matter

      Pages 111-111

    2. No Access

      Book Protocol

      Pages 113-121

      Whole-Mount Skeletal Staining

    3. No Access

      Book Protocol

      Pages 123-147

      Tips and Techniques for Processing and Sectioning Undecalcified Murine Bone Specimens

    4. No Access

      Book Protocol

      Pages 149-164

      Preparation of Thin Frozen Sections from Nonfixed and Undecalcified Hard Tissues Using Kawamot’s Film Method (2012)

    5. No Access

      Book Protocol

      Pages 165-183

      Demineralized Murine Skeletal Histology

    6. No Access

      Book Protocol

      Pages 185-191

      Beta-Galactosidase Staining in the Skeleton

    7. No Access

      Book Protocol

      Pages 193-201

      Whole-Mount In Situ Hybridization on Murine Skeletogenic Tissues

    8. No Access

      Book Protocol

      Pages 203-215

      Nonradioactive In Situ Hybridization on Skeletal Tissue Sections

    9. No Access

      Book Protocol

      Pages 217-232

      Radioactive In Situ Hybridization to Detect Gene Expression in Skeletal Tissue Sections

    10. No Access

      Book Protocol

      Pages 233-243

      Proliferation Assays (BrdU and EdU) on Skeletal Tissue Sections

    11. No Access

      Book Protocol

      Pages 245-248

      TUNEL Assay on Skeletal Tissue Sections to Detect Cell Death

  5. Primary Cell Isolations, Cultures, and Assays

    1. Front Matter

      Pages 249-249

    2. No Access

      Book Protocol

      Pages 251-265

      Analysis of Chondrogenesis Using Micromass Cultures of Limb Mesenchyme

    3. No Access

      Book Protocol

      Pages 267-277

      Isolation and Culture of Murine Primary Chondrocytes

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