Volume 1123 2014

Homing Endonucleases

Methods and Protocols

Editors:

ISBN: 978-1-62703-967-3 (Print) 978-1-62703-968-0 (Online)

Table of contents (17 protocols)

  1. Front Matter

    Pages i-x

  2. No Access

    Book Protocol

    Pages 1-26

    Homing Endonucleases: From Genetic Anomalies to Programmable Genomic Clippers

  3. No Access

    Book Protocol

    Pages 27-35

    Bioinformatic Identification of Homing Endonucleases and Their Target Sites

  4. No Access

    Book Protocol

    Pages 37-53

    PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes

  5. No Access

    Book Protocol

    Pages 55-67

    Mapping Homing Endonuclease Cleavage Sites Using In Vitro Generated Protein

  6. No Access

    Book Protocol

    Pages 69-76

    Mapping Free-Standing Homing Endonuclease Promoters Using 5′RLM-RACE

  7. No Access

    Book Protocol

    Pages 77-86

    PCR Analysis of Chloroplast Double-Strand Break (DSB) Repair Products Induced by I-CreII in Chlamydomonas and Arabidopsis

  8. No Access

    Book Protocol

    Pages 87-96

    A Two-Plasmid Bacterial Selection System for Characterization and Engineering of Homing Endonucleases

  9. No Access

    Book Protocol

    Pages 97-104

    Rapid Screening of Endonuclease Target Site Preference Using a Modified Bacterial Two-Plasmid Selection

  10. No Access

    Book Protocol

    Pages 105-126

    A Yeast-Based Recombination Assay for Homing Endonuclease Activity

  11. No Access

    Book Protocol

    Pages 127-134

    Rapid Determination of Homing Endonuclease DNA Binding Specificity Profile

  12. No Access

    Book Protocol

    Pages 135-149

    Quantifying the Information Content of Homing Endonuclease Target Sites by Single Base Pair Profiling

  13. No Access

    Book Protocol

    Pages 151-163

    Homing Endonuclease Target Site Specificity Defined by Sequential Enrichment and Next-Generation Sequencing of Highly Complex Target Site Libraries

  14. No Access

    Book Protocol

    Pages 165-190

    Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display

  15. No Access

    Book Protocol

    Pages 191-221

    Engineering and Flow-Cytometric Analysis of Chimeric LAGLIDADG Homing Endonucleases from Homologous I-OnuI-Family Enzymes

  16. No Access

    Book Protocol

    Pages 223-243

    Bioinformatics Identification of Coevolving Residues

  17. No Access

    Book Protocol

    Pages 245-264

    Identification and Analysis of Genomic Homing Endonuclease Target Sites

  18. No Access

    Book Protocol

    Pages 265-282

    Redesigning the Specificity of Protein–DNA Interactions with Rosetta

  19. Back Matter

    Pages 283-284