Volume 1119 2014

Human Cytomegaloviruses

Methods and Protocols

Editors:

ISBN: 978-1-62703-787-7 (Print) 978-1-62703-788-4 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi

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    Book Protocol

    Pages 1-14

    History of the Molecular Biology of Cytomegaloviruses

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    Book Protocol

    Pages 15-28

    Overview of Human Cytomegalovirus Pathogenesis

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    Book Protocol

    Pages 29-46

    Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies

  5. No Access

    Book Protocol

    Pages 47-57

    Use of Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegalovirus Infection

  6. No Access

    Book Protocol

    Pages 59-79

    Use of Recombinant Approaches to Construct Human Cytomegalovirus Mutants

  7. No Access

    Book Protocol

    Pages 81-98

    The Use of Primary Human Cells (Fibroblasts, Monocytes, and Others) to Assess Human Cytomegalovirus Function

  8. No Access

    Book Protocol

    Pages 99-112

    Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation

  9. No Access

    Book Protocol

    Pages 113-121

    Analysis of Cytomegalovirus Binding/Entry-Mediated Events

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    Book Protocol

    Pages 123-132

    Use of 5-Ethynyl-2′-Deoxyuridine Labelling and Flow Cytometry to Study Cell Cycle-Dependent Regulation of Human Cytomegalovirus Gene Expression

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    Book Protocol

    Pages 133-164

    Methods for Studying the Function of Cytomegalovirus GPCRs

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    Book Protocol

    Pages 165-196

    Methods for the Detection of Cytomegalovirus in Glioblastoma Cells and Tissues

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    Book Protocol

    Pages 197-216

    Methods to Study the Nucleocytoplasmic Transport of Macromolecules with Respect to Their Impact on the Regulation of Human Cytomegalovirus Gene Expression

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    Book Protocol

    Pages 217-237

    Fluorescence-Based Laser Capture Microscopy Technology Facilitates Identification of Critical In Vivo Cytomegalovirus Transcriptional Programs

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    Book Protocol

    Pages 239-265

    Techniques for Characterizing Cytomegalovirus-Encoded miRNAs

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    Book Protocol

    Pages 267-288

    What We Have Learned from Animal Models of HCMV

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    Book Protocol

    Pages 289-310

    Rodent Models of Congenital Cytomegalovirus Infection

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    Book Protocol

    Pages 311-348

    Recent Approaches and Strategies in the Generation of Antihuman Cytomegalovirus Vaccines

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    Book Protocol

    Pages 349-363

    Approaches for the Generation of New Anti-cytomegalovirus Agents: Identification of Protein–Protein Interaction Inhibitors and Compounds Against the HCMV IE2 Protein

  20. Back Matter

    Pages 365-368