Volume 1111 2014

Artificial Riboswitches

Methods and Protocols

Editors:

ISBN: 978-1-62703-754-9 (Print) 978-1-62703-755-6 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-x

  2. No Access

    Book Protocol

    Pages 1-15

    Computational Design of RNA Libraries for In Vitro Selection of Aptamers

  3. No Access

    Book Protocol

    Pages 17-28

    In Vitro Selection of RNA Aptamers for a Small-Molecule Dye

  4. No Access

    Book Protocol

    Pages 29-40

    Development of Photoswitchable RNA Aptamer–Ligand Complexes

  5. No Access

    Book Protocol

    Pages 41-56

    Identification of RNA Aptamers Against Recombinant Proteins with a Hexa-Histidine Tag

  6. No Access

    Book Protocol

    Pages 57-75

    Artificial Riboswitch Selection: A FACS-Based Approach

  7. No Access

    Book Protocol

    Pages 77-91

    FRET-Based Optical Assay for Selection of Artificial Riboswitches

  8. No Access

    Book Protocol

    Pages 93-105

    Portable Two-Way Riboswitches: Design and Engineering

  9. No Access

    Book Protocol

    Pages 107-129

    Generation of Orthogonally Selective Bacterial Riboswitches by Targeted Mutagenesis and In Vivo Screening

  10. No Access

    Book Protocol

    Pages 131-140

    Dual Genetic Selection of Synthetic Riboswitches in Escherichia coli

  11. No Access

    Book Protocol

    Pages 141-152

    Nucleotide Kinase-Based Selection System for Genetic Switches

  12. No Access

    Book Protocol

    Pages 153-164

    Measuring Riboswitch Activity In Vitro and in Artificial Cells with Purified Transcription–Translation Machinery

  13. No Access

    Book Protocol

    Pages 165-181

    Rational Design of Artificial ON-Riboswitches

  14. No Access

    Book Protocol

    Pages 183-196

    Engineering Protein-Responsive mRNA Switch in Mammalian Cells

  15. No Access

    Book Protocol

    Pages 197-207

    Guanine-Tethered Antisense Oligonucleotides as Synthetic Riboregulators

  16. No Access

    Book Protocol

    Pages 209-220

    In Vitro Selection of Allosteric Ribozymes that Sense the Bacterial Second Messenger c-di-GMP

  17. No Access

    Book Protocol

    Pages 221-235

    Dual-Selection for Evolution of In Vivo Functional Aptazymes as Riboswitch Parts

  18. No Access

    Book Protocol

    Pages 237-249

    In Vivo Screening for Aptazyme-Based Bacterial Riboswitches

  19. No Access

    Book Protocol

    Pages 251-258

    Engineered Riboswitch as a Gene-Regulatory Platform for Reducing Antibiotic Resistance

  20. No Access

    Book Protocol

    Pages 259-267

    Construction of Ligand-Responsive MicroRNAs that Operate Through Inhibition of Drosha Processing

  21. No Access

    Book Protocol

    Pages 269-286

    A Three-Dimensional Design Strategy for a Protein-Responsive shRNA Switch

  22. Back Matter

    Pages 287-289