Volume 1104 2014

Animal Cell Biotechnology

Methods and Protocols

Editors:

ISBN: 978-1-62703-732-7 (Print) 978-1-62703-733-4 (Online)

Table of contents (26 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Cell Line Development and Selection

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-13

      High-Throughput Synchronization of Mammalian Cell Cultures by Spiral Microfluidics

    3. No Access

      Book Protocol

      Pages 15-27

      Insect Cell Line Development Using Flp-Mediated Cassette Exchange Technology

    4. No Access

      Book Protocol

      Pages 29-34

      Scalable Transient Gene Expression in Adherent Mammalian Cells Using Polyethylenimine

    5. No Access

      Book Protocol

      Pages 35-55

      Scalable Transient Protein Expression

    6. No Access

      Book Protocol

      Pages 57-73

      DNA Profiling and Characterization of Animal Cell Lines

  3. Techniques for Process Development

    1. Front Matter

      Pages 75-75

    2. No Access

      Book Protocol

      Pages 77-88

      Tools for High-Throughput Process and Medium Optimization

    3. No Access

      Book Protocol

      Pages 89-103

      Designing Media for Animal Cell Culture: CHO Cells, the Industrial Standard

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      Book Protocol

      Pages 105-116

      Feed Optimization in Fed-Batch Culture

    5. No Access

      Book Protocol

      Pages 117-135

      Screening and Optimization of Chemically Defined Media and Feeds with Integrated and Statistical Approaches

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      Book Protocol

      Pages 137-147

      Evaluation of Solid and Porous Microcarriers for Cell Growth and Production of Recombinant Proteins

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      Book Protocol

      Pages 149-165

      Microbioreactors and Scale-Down Models: Growth of CHO Cells Using the Pall Micro24 MicroReactor System

  4. Cell Characterisation and Analysis

    1. Front Matter

      Pages 167-167

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      Book Protocol

      Pages 169-192

      Monitoring Cell Growth, Viability, and Apoptosis

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      Pages 193-209

      Metabolic Flux Estimation in Mammalian Cell Cultures

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      Pages 211-221

      Quenching Methods for the Analysis of Intracellular Metabolites

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      Book Protocol

      Pages 223-236

      NMR Methods for Metabolomics of Mammalian Cell Culture Bioreactors

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      Book Protocol

      Pages 237-247

      1H-NMR Protocol for Exometabolome Analysis of Cultured Mammalian Cells

  5. Cultivation Techniques

    1. Front Matter

      Pages 249-249

    2. No Access

      Book Protocol

      Pages 251-263

      Microfluidic Perfusion Culture

    3. No Access

      Book Protocol

      Pages 265-284

      Disposable Bioreactors for Inoculum Production and Protein Expression

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      Book Protocol

      Pages 285-311

      Configuration of Bioreactors

    5. No Access

      Book Protocol

      Pages 313-341

      Process Analytical Technology (PAT) in Insect and Mammalian Cell Culture Processes: Dielectric Spectroscopy and Focused Beam Reflectance Measurement (FBRM)

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