Volume 1083 2014

Plant Metabolism

Methods and Protocols

Editors:

ISBN: 978-1-62703-660-3 (Print) 978-1-62703-661-0 (Online)

Table of contents (17 protocols)

  1. Front Matter

    Pages i-x

  2. Metabolites and Metabolic Fluxes

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-16

      MeRy-B, a Metabolomic Database and Knowledge Base for Exploring Plant Primary Metabolism

    3. No Access

      Book Protocol

      Pages 17-29

      Targeted Deuteration of Polyphenolics for Their Qualitative and Quantitative Metabolomic Analysis in Plant-Derived Extracts

    4. No Access

      Book Protocol

      Pages 31-39

      Relative Quantitation in Single-Cell Metabolomics by Laser Ablation Electrospray Mass Spectrometry

    5. No Access

      Book Protocol

      Pages 41-53

      Quantification of Plant Volatiles

    6. No Access

      Book Protocol

      Pages 55-64

      Quantitative Imaging Approaches for Small-Molecule Measurements Using FRET Sensors in Plants

    7. No Access

      Book Protocol

      Pages 65-83

      Isotopomer Measurement Techniques in Metabolic Flux Analysis I: Nuclear Magnetic Resonance

    8. No Access

      Book Protocol

      Pages 85-108

      Isotopomer Measurement Techniques in Metabolic Flux Analysis II: Mass Spectrometry

    9. No Access

      Book Protocol

      Pages 109-131

      Mathematical Modeling of Isotope Labeling Experiments for Metabolic Flux Analysis

    10. No Access

      Book Protocol

      Pages 133-147

      Optimal Design of Isotope Labeling Experiments

  3. Metabolic Pathways and Metabolic Systems Biology

    1. Front Matter

      Pages 149-149

    2. No Access

      Book Protocol

      Pages 151-171

      Putting the Plant Metabolic Network Pathway Databases to Work: Going Offline to Gain New Capabilities

    3. No Access

      Book Protocol

      Pages 173-186

      Elucidation of Metabolic Pathways from Enzyme Classification Data

    4. No Access

      Book Protocol

      Pages 187-211

      Deducing Intracellular Distributions of Metabolic Pathways from Genomic Data

    5. No Access

      Book Protocol

      Pages 213-230

      Genome-Scale Models of Plant Metabolism

    6. No Access

      Book Protocol

      Pages 231-252

      Elementary Flux Modes, Flux Balance Analysis, and Their Application to Plant Metabolism

    7. No Access

      Book Protocol

      Pages 253-273

      Systems Approaches to Unraveling Plant Metabolism: Identifying Biosynthetic Genes of Secondary Metabolic Pathways

    8. No Access

      Book Protocol

      Pages 275-286

      Applications of Kinetic Modeling to Plant Metabolism

    9. No Access

      Book Protocol

      Pages 287-311

      Kinetic Modeling of Plant Metabolism and Its Predictive Power: Peppermint Essential Oil Biosynthesis as an Example

  4. Back Matter

    Pages 313-318